生腺布液汤对干燥综合征NOD小鼠B细胞活化因子及B细胞活化因子受体表达的影响OACSTPCD

Objective:To observe the effect of Shengxian Buye decoction on B-cell activating factor(BAFF)and B-cell activating factor receptor(BAFFR)in serum and submandibular gland of sjogren syndrome(SS)NOD model mice,and explore the mechanism of Shengxian Buye decoction in treating SS.Methods:248-week-old female NOD mice were randomly divided into model group,hydroxychloroquine sulfate group and Shengxian Buye decoction group,with 8 mice in each group,and 8 female BALB/c mice of the same age were selected as normal control group.Administration groups were given 1.75 g/ml of Shengxian Buye decoction and 0.0026 g/ml of hydroxychloroquine sulfate solution,respectively.Model group and normal control group were provided the same amount of distilled wa-ter for 8 weeks.During the experiment,the general situation and daily drinking water amount of mice were recorded.The pathological changes of submandibular gland of mice in each group were observed under HE staining.The ex-pression of BAFF and BAFFR in submandibular gland was detected by immunohistochemistry.The content of BAFF in serum was detected by ELISA.Results:Compared with model group,water intake of mice in Shengxian Buye de-coction group and hydroxychloroquine sulfate group decreased(all P<0.05),the expression of BAFF and BAFFR in serum and submandibular gland tissues decreased(all P<0.05),lymphocyte infiltration decreased in submandibular gland tissue,and no obvious infiltration lesions were observed.Conclusion:Shengxian Buye decoction can improve xer-ostomia and reduce lymphocyte infiltration in submandibular gland in SS NOD mice,and its mechanism may be relat-ed to reducing the expression of BAFF and BAFFR in submandibular gland and serum.