陕西中医2024，Vol.45Issue(8)：1036-1041,6.DOI:10.3969/j.issn.1000-7369.2024.08.006
生腺布液汤对干燥综合征NOD小鼠B细胞活化因子及B细胞活化因子受体表达的影响
Effect of Shengxian Buye decoction on expression of BAFF and BAFFR in NOD mice with sjogren syndrome
摘要
Abstract
Objective:To observe the effect of Shengxian Buye decoction on B-cell activating factor(BAFF)and B-cell activating factor receptor(BAFFR)in serum and submandibular gland of sjogren syndrome(SS)NOD model mice,and explore the mechanism of Shengxian Buye decoction in treating SS.Methods:248-week-old female NOD mice were randomly divided into model group,hydroxychloroquine sulfate group and Shengxian Buye decoction group,with 8 mice in each group,and 8 female BALB/c mice of the same age were selected as normal control group.Administration groups were given 1.75 g/ml of Shengxian Buye decoction and 0.0026 g/ml of hydroxychloroquine sulfate solution,respectively.Model group and normal control group were provided the same amount of distilled wa-ter for 8 weeks.During the experiment,the general situation and daily drinking water amount of mice were recorded.The pathological changes of submandibular gland of mice in each group were observed under HE staining.The ex-pression of BAFF and BAFFR in submandibular gland was detected by immunohistochemistry.The content of BAFF in serum was detected by ELISA.Results:Compared with model group,water intake of mice in Shengxian Buye de-coction group and hydroxychloroquine sulfate group decreased(all P<0.05),the expression of BAFF and BAFFR in serum and submandibular gland tissues decreased(all P<0.05),lymphocyte infiltration decreased in submandibular gland tissue,and no obvious infiltration lesions were observed.Conclusion:Shengxian Buye decoction can improve xer-ostomia and reduce lymphocyte infiltration in submandibular gland in SS NOD mice,and its mechanism may be relat-ed to reducing the expression of BAFF and BAFFR in submandibular gland and serum.关键词
干燥综合征/生腺布液汤/B细胞活化因子/B细胞活化因子受体/颌下腺/NOD小鼠Key words
Sjogren syndrome/Shengxian Buye decoction/B-cell activating factor/B-cell activating factor re-ceptor/Submandibular gland/Non obese diabetes mice分类
医药卫生引用本文复制引用
张寒,郝梦桃,王可心,张正国,艾奕锟,罗亚萍,郜晨静,张静..生腺布液汤对干燥综合征NOD小鼠B细胞活化因子及B细胞活化因子受体表达的影响[J].陕西中医,2024,45(8):1036-1041,6.基金项目
河北省重点研发计划项目(192777123D) （192777123D）
河北省老年病防治项目(2019064577) （2019064577）
