陕西中医2024，Vol.45Issue(8)：1042-1047,6.DOI:10.3969/j.issn.1000-7369.2024.08.007
艾灸联合生肌玉红膏对压疮大鼠皮肤损伤修复的作用机制
The mechanism of moxibustion combined with Shengji Yuhong ointment on the repair of skin injury in pressure ulcer rats
摘要
Abstract
Objective:To investigate the effect and mechanism of moxibustion combined with Shengji Yuhong ointment on skin injury repair in pressure ulcer(PU)rats.Methods:36 SPF SD rats were used to establish stage Ⅱ and stage Ⅲ pressure ulcer animal models by ischemia-reperfusion injury using a customized pressure ulcer modeling device.After successful modeling,they were randomly divided into model group,moxibustion group,Shengji Yuhong ointment group,and moxibustion combined with Shengji Yuhong ointment group,with 9 rats in each group.The wound healing rate was calculated on the 3rd,7th,and 14th days after continuous intervention,and the histopatholog-ical conditions were observed.The expression levels of phosphorylated PI3 K(p-PI3 K),phosphorylated AKT(p-Akt),and phosphorylated mTOR(p-mTOR)proteins in wound tissues were detected by immunohistochemistry.Results:On the 7th and 14th days after treatment,the wound healing rate of the moxibustion combined with Shengji Yuhong ointment group was higher than that of Shengji Yuhong ointment group,moxibustion group and model group(all P<0.05).HE staining showed that there was less inflammatory cell infiltration in the moxibustion combined with Shengji Yuhong ointment group,and more new granulation tissue and a large number of closely arranged fibro-blasts were observed.The results of immunohistochemistry showed that the expression levels of p-PI3 K,p-Akt and p-mTOR proteins began to increase from the 3rd day,reached the highest on the 7th day,and then gradually de-creased.On 7th and 14th days after intervention,the protein expressions of p-PI3 K,p-Akt and p-mTOR in moxibus-tion combined with Shengji Yuhong ointment group were higher than those in moxibustion group,Shengji Yuhong ointment group and model group(all P<0.05).Conclusion:Moxibustion combined with Shengji Yuhong ointment can significantly promote wound repair and improve pathological tissue morphology in rats with pressure sores.The mechanism may be related to the activation of PI3 K/Akt/mTOR signaling pathway,promotion of angiogenesis and protein synthesis,and anti-apoptosis.关键词
压疮/皮肤损伤修复/艾灸/生肌玉红膏/磷脂酰肌醇-3激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白Key words
Pressure ulcer/Skin injury repair/Moxibustion/Shengji Yuhong ointment/Phosphatidylinositol-3 kinase/Protein kinase B/Mammalian rapamycin target protein分类
医药卫生引用本文复制引用
陈若岚,薛慧琴,丁劲,葸英博,刘静,刘雪丽,王霞..艾灸联合生肌玉红膏对压疮大鼠皮肤损伤修复的作用机制[J].陕西中医,2024,45(8):1042-1047,6.基金项目
甘肃省自然科学基金资助项目(21JR11RA154) （21JR11RA154）
