艾灸联合生肌玉红膏对压疮大鼠皮肤损伤修复的作用机制OACSTPCD
The mechanism of moxibustion combined with Shengji Yuhong ointment on the repair of skin injury in pressure ulcer rats
目的:探讨艾灸联合生肌玉红膏对压疮大鼠皮肤损伤修复的影响及作用机制.方法:36 只 SPF级 SD大鼠均采用定制压疮造模装置通过缺血-再灌注损伤方式建立Ⅱ、Ⅲ期压疮动物模型,判断造模成功后随机分为模型组、艾灸组、生肌玉红膏组、艾灸联合生肌玉红膏组,每组 9 只,分别于连续干预 3、7、14 d 计算创面愈合率,观察组织病理情况,采用免疫组化法检测创面组织磷酸化PI3 K(p-PI3 K)、磷酸化 AKT(p-Akt)、磷酸化 mTOR(p-mTOR)蛋白表达水平.结果:干预 7、14 d,艾灸联合生肌玉红膏组创面愈合率高于生肌玉红膏组、艾灸组、模型组(均P<0.05).HE染色发现,艾灸联合生肌玉红膏组炎性细胞浸润较少,可见较多新生肉芽组织及大量排列紧密的成纤维细胞.免疫组化结果显示,p-PI3 K、p-Akt、p-mTOR蛋白表达水平从干预 3 d开始升高,干预 7 d达到高点,随后逐渐降低.干预 7、14 d,艾灸联合生肌玉红膏组 p-PI3 K、p-Akt、p-mTOR蛋白表达高于艾灸组、生肌玉红膏组及模型组(均P<0.05).结论:艾灸联合生肌玉红膏可显著促进压疮大鼠创面修复,改善病理组织形态,其作用机制可能与激活PI3 K/Akt/mTOR信号通路,促进血管新生及蛋白质合成,发挥抗凋亡作用有关.
Objective:To investigate the effect and mechanism of moxibustion combined with Shengji Yuhong ointment on skin injury repair in pressure ulcer(PU)rats.Methods:36 SPF SD rats were used to establish stage Ⅱ and stage Ⅲ pressure ulcer animal models by ischemia-reperfusion injury using a customized pressure ulcer modeling device.After successful modeling,they were randomly divided into model group,moxibustion group,Shengji Yuhong ointment group,and moxibustion combined with Shengji Yuhong ointment group,with 9 rats in each group.The wound healing rate was calculated on the 3rd,7th,and 14th days after continuous intervention,and the histopatholog-ical conditions were observed.The expression levels of phosphorylated PI3 K(p-PI3 K),phosphorylated AKT(p-Akt),and phosphorylated mTOR(p-mTOR)proteins in wound tissues were detected by immunohistochemistry.Results:On the 7th and 14th days after treatment,the wound healing rate of the moxibustion combined with Shengji Yuhong ointment group was higher than that of Shengji Yuhong ointment group,moxibustion group and model group(all P<0.05).HE staining showed that there was less inflammatory cell infiltration in the moxibustion combined with Shengji Yuhong ointment group,and more new granulation tissue and a large number of closely arranged fibro-blasts were observed.The results of immunohistochemistry showed that the expression levels of p-PI3 K,p-Akt and p-mTOR proteins began to increase from the 3rd day,reached the highest on the 7th day,and then gradually de-creased.On 7th and 14th days after intervention,the protein expressions of p-PI3 K,p-Akt and p-mTOR in moxibus-tion combined with Shengji Yuhong ointment group were higher than those in moxibustion group,Shengji Yuhong ointment group and model group(all P<0.05).Conclusion:Moxibustion combined with Shengji Yuhong ointment can significantly promote wound repair and improve pathological tissue morphology in rats with pressure sores.The mechanism may be related to the activation of PI3 K/Akt/mTOR signaling pathway,promotion of angiogenesis and protein synthesis,and anti-apoptosis.
陈若岚;薛慧琴;丁劲;葸英博;刘静;刘雪丽;王霞
甘肃中医药大学,甘肃 兰州 730000||甘肃中医药大学附属医院,甘肃 兰州 730000甘肃中医药大学附属医院,甘肃 兰州 730000甘肃中医药大学,甘肃 兰州 730000
临床医学
压疮皮肤损伤修复艾灸生肌玉红膏磷脂酰肌醇-3激酶蛋白激酶B哺乳动物雷帕霉素靶蛋白
Pressure ulcerSkin injury repairMoxibustionShengji Yuhong ointmentPhosphatidylinositol-3 kinaseProtein kinase BMammalian rapamycin target protein
《陕西中医》 2024 (008)
1042-1047 / 6
甘肃省自然科学基金资助项目(21JR11RA154)
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