上海农业学报2024，Vol.40Issue(3)：1-7,7.DOI:10.15955/j.issn1000-3924.2024.03.01
转基因抗除草剂大豆ZH10-6转化体特异性定量检测体系的建立
Establishing an specific quantitative detection system for transgenic herbicide-resistant soybean ZH10-6 transformant
摘要
Abstract
A precise quantitative detection system for transformant specific real-time fluorescence PCR and single channel micro drop digital PCR was established using the flanking sequence of the exogenous insertion fragment of the novel genetically modified herbicide-resistant soybean ZH10-6 as the target.Eight testing centers of the Ministry of Agriculture and Rural Affairs were invited to verify the accuracy of the detection method.The results showed that the transformant specific real-time fluorescence quantitative detection method had good specificity,repeatability,and wide applicability,with a quantitative limit of 50 copies/μL and a detection limit of 5 copies/μL.This detection method was expected to serve as a detection standard for the quantitative detection of genetically modified herbicide-resistant soybean ZH10-6,providing technical support for the commercial application and regulation of genetically modified labeling of this soybean variety(line).关键词
抗除草剂大豆ZH10-6/转化体特异性/定量检测/实时荧光PCR/微滴式数字PCRKey words
Herbicide-resistant soybean ZH10-6/Transformant specific/Quantitative detection/Real-time PCR/Droplet digital PCR分类
农业科技引用本文复制引用
白蓝,贾军伟,陈一帆,章寅,潘爱虎,吴潇..转基因抗除草剂大豆ZH10-6转化体特异性定量检测体系的建立[J].上海农业学报,2024,40(3):1-7,7.基金项目
上海市农业科学院卓越团队建设计划项目[沪农科卓(2022)016] （2022）
