上海农业学报2024，Vol.40Issue(3)：8-12,5.DOI:10.15955/j.issn1000-3924.2024.03.02
基于ISSR分子标记的27份菊科种质遗传多样性分析
Genetic diversity analysis of 27 compositae germplasms based on ISSR molecular markers
摘要
Abstract
In order to preserve and identify compositae germplasm resources,ISSR markers were used to analyze the genetic diversity and genetic relationship of 27 compositae germplasms.The polymorphisms were analyzed by NTSYS-pc 2.1,the genetic tree was constructed by UPGMA.The results showed that 10 primers were screened and 255 clear polymorphic bands were amplified,accounting for 94.80％of the total bands.The UPGMA cluster analysis showed that the genetic similarity coefficients of 27 compositae germplasms ranged from 0.36 to 1.00,and the 27 compositae germplasms could be divided into three categories at 0.40 similarity coefficient.The 27 compositae germplasms have rich genetic diversity and the 10 ISSR primers can be used for molecular identification of germplasm resources.关键词
菊科/亲缘关系/ISSR/聚类分析/遗传多样性Key words
Compositae/Genetic relationship/ISSR/Cluster analysis/Genetic diversity分类
农业科技引用本文复制引用
李秋静,申瑞雪,黄建荣..基于ISSR分子标记的27份菊科种质遗传多样性分析[J].上海农业学报,2024,40(3):8-12,5.基金项目
上海市花卉产业技术体系建设专项[沪农科产字(2022-2026)第8号] （2022-2026）
