上海海洋大学学报2024，Vol.33Issue(4)：823-835,13.DOI:10.12024/jsou.20240404528
基于CO Ⅰ基因构建西北太平洋常见鱼类DNA条形码参考数据库
Construction of DNA barcode reference database of common fish in Northwest Pacific Ocean based on CO Ⅰ gene
摘要
Abstract
With the unique geography and complex environment,the Northwest Pacific Ocean has nurtured extremely rich fishery resources,becoming a hotspot for global marine biodiversity conservation and fishery management.In order to more effectively identify and investigate the diversity of fish species,a local DNA barcodes database of common fish in Northwest Pacific Ocean was established.Based on the CO Ⅰ gene sequences of fish,307 samples of common fish were collected in Northwest Pacific Ocean from June to August 2023.These common fish belonged to 20 genera,13 families,7 orders,and 25 fish species.77.96％of COⅠ sequences can be compared to high similarity sequences in public database.The average interspecific genetic distance was 0.233,and the average intraspecific genetic distance was 0.003,and the interspecific genetic distance was 77.67 times that of the intraspecific genetic distance,and it could form obvious bar code gap.The phylogenetic tree based on CO Ⅰ gene sequences showed that fishes of the same genus first clustered into a single branch,then with fishes of the same family,and finally with fish of different families of the same order.In summary,the CO Ⅰ gene has species specificity and can effectively distinguish common fish species in the Northwest Pacific Ocean.The initial establishment of the DNA barcode database of common fish is conducive to the monitoring and investigation of fish diversity in Northwest Pacific Ocean using environmental DNA technology,providing technical support for biodiversity conservation,resource management,and population dynamic monitoring in Northwest Pacific Ocean.关键词
鱼类/CO Ⅰ基因/DNA条形码数据库/西北太平洋Key words
fish/CO Ⅰ gene/DNA barcode database/Northwest Pacific Ocean分类
农业科技引用本文复制引用
徐颖琪,梁绪虹,陈新军,宋成辉,彭祖焜,王丛丛..基于CO Ⅰ基因构建西北太平洋常见鱼类DNA条形码参考数据库[J].上海海洋大学学报,2024,33(4):823-835,13.基金项目
农业农村部全球渔业资源调查监测评估(公海渔业资源综合科学调查)专项(D-8025-23-1002) （公海渔业资源综合科学调查）
上海市高校特聘教授"东方学者"岗位跟踪计(GZ2022011) （GZ2022011）
