上海针灸杂志2024，Vol.43Issue(7)：791-798,8.DOI:10.13460/j.issn.1005-0957.2024.07.0791
电针对功能性消化不良大鼠肠道菌群的影响
Effect of electroacupuncture on intestinal microbiota in functional dyspepsia rats
摘要
Abstract
Objective To observe the effect of electroacupuncture on intestinal microbiota in functional dyspepsia(FD)rats.Method Forty male SD rats were randomized into a blank group(10 rats)and a modeling group(30 rats).Rats in the modeling group were prepared as FD models using a multi-factor combination method.Twenty successful models were then randomly divided into a model group and an electroacupuncture group,each consisting of 10 rats.The electroacupuncture group received acupuncture at Zusanli(ST36)and Taichong(LR3)with Zusanli connected with the electroacupuncture device.The intervention lasted 14 d.Each group was observed for changes in the rat's body mass,gastric residual rate,and small intestine propulsion rate,and the 16SrRNA sequencing technique was used to analyze changes in the rat's intestinal microbiota.Result Compared with the blank group,the rat's body mass grew more slowly,the gastric residual rate increased,and the intestine propulsion rate dropped in the model group;after the intervention,the rat's body mass increased,the gastric residual rate decreased,and the intestine propulsion rate increased in the electroacupuncture group compared to the model group(P＜0.01).The Adonis,NMDS,and PCoA showed significant differences between the blank and model groups when comparing the rat's intestinal microbiota structural composition,and the intestinal microbiota structure changed in the model group rats.Chao1 index and Shannon index revealed that there were insignificant differences among the three groups in comparing the total number of species,though significant differences existed between the model and blank groups in the diversity and uniformity of intestinal microbiota,and the electroacupuncture group was closer to the blank group in comparing the diversity and uniformity of intestinal microbiota.The fecal samples lacked Acidobacteria in the model group rats,while electroacupuncture increased the relative abundance of Acidobacteria in FD rats,more approaching to that in the blank group.Conclusion FD model rats have significant differences in the intestinal microbiota structure compared to normal rats,and the intestinal microbiota structure and species composition have undergone changes in FD rats.Electroacupuncture can regulate the gut microbiota structure in FD rats and improve their gastrointestinal motility.关键词
针刺疗法/电针/功能性消化不良/肠道菌群/大鼠Key words
Acupuncture therapy/Electroacupuncture/Functional dyspepsia/Intestinal microbiota/Rats分类
医药卫生引用本文复制引用
康朝霞,司原成,韩永丽,张二伟,崔冬梅..电针对功能性消化不良大鼠肠道菌群的影响[J].上海针灸杂志,2024,43(7):791-798,8.基金项目
国家自然科学基金项目(82060897) （82060897）
贵州省科技计划项目[黔科合基础-ZK(2022)一般466] （2022）
贵州省中医药管理局中医药、民族医药科学技术研究项目(QZYY-2022-007) （QZYY-2022-007）
贵州省教育厅高等学校科学研究项目青年项目[黔科技(2022)211] （2022）
贵州中医药大学博士启动基金[贵中医博士启动(2020)27号] （2020）
