麒麟竭果实化学成分及其抑制血小板活性成分的研究OA北大核心CHSSCDCSTPCD

Objective:To investigate the chemical constituents in the ethyl acetate fraction of the fruit extract of Daemonorops draco Bl.and their platelet-inhibitory activities.Methods:The ethyl acetate fraction of the fruit extract of D.draco was separated and pu-rified by column chromatography with silica gel,ODS,and Sephadex LH-20 and semi-preparative HPLC.The structures of the chemical constituents were identified based on the spectral data from 1 H-NMR,l3C-NMR,and MS.The ATP release from thrombin-activated platelets was employed to screen the active constituents.Results:Eleven compounds were obtained from the ethyl acetate fraction of the fruit extract of D.draco.They were identified as ethyl 4,6-dihydroxy-2-methoxy-3-methylbenzoate(1),6-hydroxy-2,4-dimethoxy-3-methyl phenylethanol(2),4,6-dihydroxy-2-methoxy-3-methyl benzaldehyde(3),ethyl p-hydroxy benzoate(4),methyl 4-hydroxy benzoate(5),3,5-dichlorosalicylic acid(6),p-hydroxybenzaldehyde(7),pinocembrin(8),strobopinin(9),7-hy-droxy-5-methoxy-6-methylflavanone(10),and ozoroalide(11).Conclusion:Compound 1 was a new compound and compounds 2 and 3 were new natural products.Compounds 3,7,and 9 significantly inhibited the ATP release from thrombin-activated platelets.