不同剂量附子水煎液对慢性心力衰竭小鼠的干预作用OA北大核心CHSSCDCSTPCD

Objective:To investigate the effects of different doses of aconite decoction on mice with chronic heart failure(CHF).Methods:Fifty-three healthy male C57/6J mice were randomly divided into a control group,a model group,and low,middle,and high-dose aconite decoction groups.CHF mouse models were induced by intraperitoneal injection of doxorubicin(Dox).The mice were treated with aconite decoction at different concentrations(0.2,0.6,2 mg/kg)via intragastric administration once daily for 30 consecutive days.The survival rate and general indexes were recorded,cardiac function was evaluated,and histopathological chan-ges in myocardial tissue were observed.Serum levels of brain natriuretic peptide(BNP)and cardiac troponin Ⅰ(cTnⅠ)were detec-ted.The mRNA expression levels of atrial natriuretic peptide(ANP)and BNP were detected.Additionally,expression levels of acti-vated aspartate-specific caspase(cleaved-caspase-3)and apoptotic protein Bax(Bcl2-associated X protein,Bax)were detected.Re-sults:Compared with the model group,the cardiac function of mice in all aconite decoction groups improved(P＜0.05),with the most significant improvement observed in the high-dose aconite decoction group,as indicated by an increase in myocardial tissue cross-section.Serum BNP and cTnⅠ levels decreased(P＜0.05).The mRNA expression level of BNP and the protein expression levels of cleaved-caspase-3 and Bax significantly decreased(P＜0.05).Conclusion:Aconite decoction inhibits myocardial cell ap-optosis in a dose-dependent manner,reduces the changes in cardiac function and pathological structure in mice,and thereby im-proves Dox-induced CHF.