世界中医药2024，Vol.19Issue(10)：1406-1413,8.DOI:10.3969/j.issn.1673-7202.2024.10.006
三黄益肾胶囊调控NF-κB信号通路减轻糖尿病肾病大鼠炎症反应的机制研究
The Mechanism of Sanhuang Yishen Capsules in Alleviating Inflammatory Response in Diabetic Nephropathy Rats by Regulating the Nuclear Factor-KB Signaling Pathway
摘要
Abstract
Objective:To explore the potential inflammatory response mechanism of the Sanhuang Yishen Capsules in the treatment of diabetic nephropathy(DN).Methods:Sixty rats were divided into the normal group,model group,irbesartan group,and low,medium,and high dose Sanhuang Yishen Capsules groups according to the random number table method,with 10 rats in each group.Except for the nor-mal group,the other groups were fed a high-sugar and high-fat diet combined with streptozotocin(STZ)injection to establish a DN mod-el.The normal and model groups were given 2 mL of normal saline via gavage;the irbesartan group was given 11.51 mg/kg of irbesartan via gavage;and the low,medium,and high dose Sanhuang Yishen Capsules groups were given 0.41,0.81,and 1.62 g/kg of Sanhuang Yishen Capsules via gavage,respectively.All groups received the treatments once daily for 4 consecutive weeks.The following indexes a-mong the groups were compared among the groups:fasting blood glucose(FBG),body weight,24-hour urine albumin,renal function indi-cators,renal histopathological changes,renal tissue oxidative stress-related indicators(SOD,GSH-Px activity,and MDA levels).The ex-pression levels of renal tissue inflammatory mediators[Interleukin-6(IL-6),interleukin-1β(IL-1 β),tumor necrosis factor-a(TNF-a)],renal tissue inducible nitric oxide synthase(iNOS),renal tissue CD68,CC chemokine receptor 2(CCR2),and renal tissue CC chemokine ligand 2(CCL2),NF-κB P65,p-NF-κB P65,IκB kinase(IKB),p-IκB were also compared among the groups.Results:Compared with the model group,rats in the Sanhuang Yishen Capsules groups had higher body weight and lower levels of FBG,serum creatinine(Cr),blood urea nitrogen(BUN),and 24-hour urine protein.The renal histopathological changes were alleviated,and the activities of SOD and GSH-Px in renal tissues were higher,while the MDA levels were lower.Levels of IL-6,IL-1 β,TNF-a,and iNOS in renal tissues were lower.The co-localization level of CCR2 and CD68 in renal tissues was lower,as well as the expressions of CCL2,p-NF-KB P65,and p-IκB proteins.Conclusion:The mechanism of Sanhuang Yishen Capsules in the treatment of DN may be related to the inhibition of NF-κB signaling pathway activation in renal tissues,and the reduction of renal oxidative stress and inflammatory response.关键词
三黄益肾胶囊/糖尿病肾病/炎症反应/大鼠/CC趋化因子配体2/CC趋化因子受体2信号通路/转录因子κB信号通路/肾功能/血糖Key words
Sanhuang Yishen Capsules/Diabetic nephropathy/Inflammatory response/Rat/CC chemokine ligand 2(CCL2)/CC chemokine receptor 2(CCR2)signaling pathway/Nuclear Factor-KB(NF-κB)signaling pathway/Renal function/Blood glucose分类
临床医学引用本文复制引用
吕树泉,苏秀海,潘保朝,刘爱茹,孙文娟,刘晓菲,杨越,王丛香,李函舟,崔换天..三黄益肾胶囊调控NF-κB信号通路减轻糖尿病肾病大鼠炎症反应的机制研究[J].世界中医药,2024,19(10):1406-1413,8.基金项目
国家自然科学基金项目(82274424)——基于"痰浊内阻"病机研究二陈汤通过调节甘油磷脂代谢启动线粒体自噬治疗非酒精性脂肪性肝病的作用机制 （82274424）
河北省自然科学基金项目(H2022110019)——基于"胱氨酸/GSH/GPX4轴"研究三黄益肾胶囊抑制肾小管上皮细胞铁死亡治疗糖尿病肾病的作用机制 （H2022110019）
