新外来中药辣木叶对虚热及虚寒证候模型生物学表达特征的影响OA北大核心CHSSCDCSTPCD

Objective:To investigate the biological expression characteristics of Moringa spp.leaves on mouse models of deficien-cy-heat syndrome and deficiency-cold syndrome.Methods:Kunming male mice were divided into blank group,deficiency-heat mod-el group,deficiency-heat group treated with Panax quinquefolius,deficiency-heat groups treated with Moringa spp.leaves(high,me-dium,low doses),deficiency-cold model group,deficiency-cold group treated with P.ginseng,and deficiency-cold groups treated with Moringa spp.leaves(high,medium,low doses).Thyroxine tablet solution and hydrocortisone solution were used to prepare the models of deficiency-heat and deficiency-cold syndromes,respectively.Enzyme-linked immunosorbent assay(ELISA)was used to measure the serum levels of cyclic adenosine monophosphate(cAMP),cyclic guanosine monophosphate(cGMP),cAMP/cGMP ra-tio,triiodothyronine(T3),thyroxine(T4),interleukin-6(IL-6),and interleukin-1 β(IL-1 β).Results:Compared with the deficiency-heat model group,the deficiency-heat groups treated with P.quinquefolius and Moringa spp.leaves showed significantly decreased levels of cAMP,cAMP/cGMP ratio,T3,T4,IL-6,and IL-1 β.Compared with the deficiency-cold model group,the deficiency-cold group treated with P.ginseng showed significantly increased levels of cAMP,cAMP/cGMP ratio,T3,T4,and decreased IL-6 and IL-1β.The deficiency-cold groups treated with Moringa spp.leaves showed no significant change in cAMP and cAMP/cGMP ratio,but significantly decreased levels of T3,T4,IL-6,and IL-1 β.Conclusion:The study found that Moringa spp.leaves had similar mecha-nisms of action to P.quinquefolius,exhibiting cold properties,contrary to the action of P.ginseng.At the level of immune factors,Moringa spp.leaves demonstrated bidirectional regulatory effects,possibly influenced by different chemical compositions,showing immune-modulating effects in deficiency-cold and deficiency-heat mouse models.Considering the levels of cyclic nucleotide metabo-lism and hormone levels,Moringa spp.leaves exhibited properties tending to cold or cool,consistent with the theoretical analysis of their medicinal properties.