新外来中药紫锥菊对虚热证虚寒证小鼠甲状腺激素和物质能量代谢的影响OA北大核心CHSSCDCSTPCD

Objective:To investigate the effects of water extract of Echinacea purpurea on the endocrine hormones and material and energy metabolism in mice with deficiency-cold and deficiency-heat syndrome and explore the pharmacological characteristics of E.purpurea.Methods:Male KM mice were randomly divided into blank group,deficiency-heat model group,deficiency-heat Panax quinquefolius group,deficiency-heat low-dose E.purpurea extract group,deficiency-heat high-dose E.purpurea extract group,defi-ciency-cold model group,deficiency-cold P.ginseng group,deficiency-cold low-dose E.purpurea extract group,and deficiency-cold high-dose E.purpurea extract group.Mice in the deficiency-heat model group were administered water solution of thyroid tablets(160 mg/kg)orally daily,while those in the deficiency-cold model group received hydrocortisone solution(25 mg/kg)orally once daily for 14 consecutive days to establish the deficiency-heat and deficiency-cold models,respectively.Each treatment group re-ceived corresponding drugs orally.Serum levels of triiodothyronine(T3),thyroxine(T4),total cholesterol(TC),triglycerides(TG),total protein(TP),albumin(ALB),and glucose(GLU)were measured.The activities of lactate dehydrogenase(LDH),succinate dehydrogenase(SDH),and Na+-K+-ATP in the liver were assessed using colorimetric methods.Real-time polymerase chain reac-tion(PCR)was used to detect mRNA expression of cytochrome c oxidase subunit 7A2(COX7A2)and succinate dehydrogenase complex subunit D(SDHD)in liver tissue.Results:Compared to the deficiency-heat model group,E.purpurea extract improved the overall condition of deficiency-heat mice,with improvements in body weight and spleen index(P＜0.05).Levels of T3 and T4 were significantly reduced(P＜0.05,P＜0.01),while levels of TP,ALB,GLU,and LDH were significantly increased(P＜0.01).Ex-pression levels of SDH,Na+-K+-ATP,COX7 A2,and SDHD mRNA were significantly decreased(P＜0.05,P＜0.01).E.purpurea extract exhibited similar effects to P.quinquefolius with cold-nature properties.Compared to the deficiency-cold model group,E.pur-purea extract showed no significant improvement in the overall condition,thymus index,or spleen index of deficiency-cold mice.However,high-dose E.purpurea extract significantly increased SDH and Na+-K+-ATP levels(P＜0.01).There was no significant modulation of T3,T4,TP,ALB,TC,TG,GLU,COX7A2 mRNA,or SDHD mRNA expression levels in deficiency-cold mice treated with E.purpurea extract(P＞0.05).E.purpurea extract exhibited effects contrary to P.ginseng with warm-nature properties.Con-clusion:E.purpurea extract exhibits a cool-nature property,which may be the material basis for its cool-nature pharmacological characteristics.