世界中医药2024，Vol.19Issue(12)：1737-1742,6.DOI:10.3969/j.issn.1673-7202.2024.12.005
益肺宣肺降浊方通过影响脑源性神经营养因子改善血管痴呆记忆的作用机制
Yifei Xuanfei Jiangzhuo Formula Improves Memory in Vascular Dementia by Regulating Brain-derived Neurotrophic Factor
摘要
Abstract
Objective:To investigate the role and mechanism of Yifei Xuanfei Jiangzhuo Formula in improving memory in vascular dementia by regulating brain-derived neurotrophic factor(BDNF).Methods:Wistar rats were randomly assigned into sham,model,and low-,medium-,high-dose(1.25,2.5,and 5 g/kg,respectively)Yifei Xuanfei Jiangzhuo Formula,and huperzine A(3 mg/kg)groups(n=15).The rats in other groups except the sham group were subjected to middle cerebral artery occlusion(MCAO)for the modeling of vascular dementia.The rats in the drug intervention groups were administrated with corresponding drugs by gavage,and those in the sham and model groups were administrated with the same volume of normal saline once a day for 4 weeks.Morris water maze test was performed to examine the neurobehaviors of the rats.After the test,the hippocampal tissue was collected,and the ter-minal-deoxy nucleoitidyl transferase mediated nick end labeling(TUNEL)was employed to detect apoptosis in the hippocampal tis-sue.The BDNF expression in the hippocampal tissue was determined by immunohistochemistry.Results:The results of Morris water maze test showed that compared with the model group,Yifei Xuanfei Jiangzhuo Formula shortened the escape latency(P＜0.05)and increased the number of crossing the platform(P＜0.05).The TUNEL results showed that Yifei Xuanfei Jiangzhuo Formula in-hibited the apoptosis of hippocampal neurons(P＜0.05).The immunohistochemical results showed that Yifei Xuanfei Jiangzhuo Formula up-regulated the BDNF expression in the hippocampal tissue(P＜0.05).Conclusion:Yifei Xuanfei Jiangzhuo Formula can improve the learning and memory in the rat model of vascular dementia by inhibiting neuronal apoptosis and up-regulating BD-NF expression.关键词
益肺宣肺降浊方/血管痴呆/脑源性神经营养因子/海马/神经元/凋亡Key words
Yifei Xuanfei Jiangzhuo Formula/Vascular dementia/Brain-derived neurotrophic factor/Hippocampus/Neurons/Apoptosis分类
中医学引用本文复制引用
陈炜,匡龙娇,胡跃强,吴林,朱健敏,袁炳茂,洪煌钟,朱小敏,蒋凌飞..益肺宣肺降浊方通过影响脑源性神经营养因子改善血管痴呆记忆的作用机制[J].世界中医药,2024,19(12):1737-1742,6.基金项目
国家自然科学基金地区科学基金项目(81760847,82060844) （81760847,82060844）
广西自然科学基金重点项目(2018GXNSFDA050018) （2018GXNSFDA050018）
广西自然科学基金项目(2022GXNSFBA035663) （2022GXNSFBA035663）
广西中医药大学一流学科开放课题(2019XK018) （2019XK018）
2020年广西高等学校高水平创新团队及卓越学者计划(桂教人才[2020]6号) （桂教人才[2020]6号）
广西中医脑病临床研究中心(桂科AD20238028) （桂科AD20238028）
广西中医药重点学科建设项目(GZXK-Z-20-13) （GZXK-Z-20-13）
