玄参提取物对糖尿病视网膜病变微血管内皮细胞损伤的影响及其作用机制OA北大核心CHSSCDCSTPCD
Scrophulariae Radix Extract Alleviates the Damage of Microvascular Endothelial Cells in Diabetic Retinopathy
目的:探讨玄参提取物抑制微小RNA-646(miR-646)改善微血管内皮细胞损伤缓解糖尿病视网膜水肿的影响及其作用机制.方法:根据简单随机化法将人视网膜微血管内皮细胞(HRMEC)细胞分为对照组、模型组(30 mmol/L葡萄糖)、观察组(30 mmol/L葡萄糖+100 μg/mL玄参提取物组)、模型+Anti-NC(转染Anti-NC+30 mmol/L葡萄糖)、模型+Anti-miR-646(转染Anti-miR-646+30 mmol/L葡萄糖)组;将30只小鼠根据简单随机化法分为对照组、模型组(60 mg/kg的链脲佐菌素)、观察组(60 mg/kg的链脲佐菌素+100 mg/kg-的玄参提取物),每组10只.比较各组细胞增殖,迁移及miR-646、血管内皮生长因子A(VEGFA)、细胞间黏附分子-1(ICAM-1)、血小板-内皮细胞黏附分子(CD31)表达;比较各组小鼠视网膜组织ICAM-1、CD31表达及视网膜组织水肿情况.结果:细胞实验结果显示,与对照组比较,模型组细胞miR-646、ICAM-1表达显著升高,VEGFA、CD31表达显著下降(均P＜0.05);与模型组比较,观察组细胞miR-646、ICAM-1表达显著降低,VEGFA、CD31表达显著升高(均P＜0.05);与模型+Anti-NC组比较,模型+Anti-miR-646组细胞增殖率、迁移细胞数及VEGFA表达显著增加,miR-646表达及凋亡率显著降低(均P＜0.05).动物实验结果显示,与对照组比较,模型组小鼠视网膜组织中miR-646、ICAM-1表达显著升高,VEGFA、CD31表达显著下降(均P＜0.05),同时小鼠视网膜组织水肿情况加重;与模型组比较,观察组小鼠网膜组织中miR-646、ICAM-1表达显著降低,VEGFA、CD31表达显著升高(均P＜0.05),同时小鼠视网膜组织水肿明显得到缓解.结论:玄参提取物可能通过miR-646/VEGFA机制调节HRMEC细胞增殖、迁移和凋亡,改善缓解糖尿病视网膜水肿.
Objective:To investigate the effects of Scrophulariae Radix extract on the damage of human retinal microvascular endo-thelial cells(HRMECs)in diabetic retinopathy(DR)and decipher the underlying mechanism.Methods:The simple random meth-od was adopted to group HRMECs:control,model(30 mmol/L glucose),observation(30 mmol/L glucose+100 µg/mL Scrophular-iae Radix extract),model+anti-NC(transfected with anti-NC+30 mmol/L glucose),and model+anti-miR-646(transfected with anti-miR-646+30 mmol/L glucose).Thirty mice were assigned into control,model(60 mg/kg streptozotocin),and observation(60 mg/kg streptozotocin+100 mg/kg Scrophularia Radix extract)groups(n=10)according to the simple random method.Cell proliferation,migration,and expression of miR-646,vascular endothelial growth factor A(VEGFA),intercellular adhesion molecule-1(ICAM-1),and platelet endothelial cell adhesion molecule-1(CD31)were compared among different cell groups.In addition,the expression of ICAM-1 and CD31 in the retinal tissue and the degree of retinal edema were compared among different mouse groups.Results:In the cell experiment,compared with control group,the model group presented upregulated expression of miR-646 and ICAM-1 and downregulated expression of VEGFA and CD31(all P＜0.05).Compared with the model group,the observation group exhibited downregulated expression of miR-646 and ICAM-1 and upregulated expression of VEGFA and CD31(all P＜0.05).Com-pared with the model+anti-NC group,the model+anti-miR-646 group showcased increased cell proliferation rate,migrated cells,and VEGFA expression,downregulated miR-646 expression,and decreased apoptosis rate(all P＜0.05).In the animal experiment,compared with the control group,the model group showcased upregulated expression of miR-646 and ICAM-1,downregulated ex-pression of VEGFA and CD31 in the retinal tissue(all P＜0.05),and aggravated retinal edema.Compared with model group,the observation group demonstrated downregulated expression of miR-646 and ICAM-1,upregulated expression of VEGFA and CD31 in the retinal tissue(all P＜0.05),and relieved retinal edema.Conclusion:Scrophulariae Radix extract may regulate the prolifera-tion,migration,and apoptosis of HRMECs via miR-646/VEGFA to promote the remission of retinal edema in diabetic retinopathy.
阚悦铭;张珊珊
山东中医药大学第一临床医学院,济南,250355山东中医药大学第一附属医院内分泌科,济南,250355
临床医学
糖尿病视网膜病变糖尿病视网膜水肿玄参提取物miR-646视网膜微血管内皮细胞增殖凋亡迁移
Diabetic retinopathyDiabetic retinal edemaScrophulariae Radix extractmiR-646Retinal microvascular endothe-lial cellsProliferationApoptosisMigration
《世界中医药》 2024 (012)
1757-1765 / 9
山东省老年医学学会2021年科技攻关项目(LKJGG2021Y0420)
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