金昭胶囊对慢性酒精性肝损伤小鼠的保护作用OA北大核心CHSSCDCSTPCD

Objective:To decipher the mechanism of Jinzhao Capsules in protecting mice from chronic alcoholic liver injury via the Kelch-like ECH-associated protein-1/nuclear factor erythroid 2-related factor 2/nuclear factor-κB(Keap1/Nrf2/NF-κB)pathway.Methods:Mice were randomized into blank,model,positive drug,and low-,medium-,and high-dose Jinzhao Capsules groups(n=10)according to their body weights.The mice in the other groups except the blank group were administrated with 50％ethanol at a dose of 10 mL/kg by gavage every day.After four consecutive weeks of drug administration,the status of mice was observed,and the activity of transaminase in the serum as well as the levels of ethanol,fat metabolism-related enzymes,and antioxidant enzymes in the liver were measured.In addition,the protein and mRNA levels of factors in the Keap1/Nrf2/NF-κB pathway were determined.The liver tissue was sampled and stained with hematoxylin and eosin,and the pathological changes were examined.Results:Com-pared with the model group,Jinzhao Capsules groups showed increased transaminase activity in the serum,elevated levels of etha-nol,fat metabolism-related enzymes,antioxidant enzymes,and interleukin-10(IL-10)in the liver,up-regulated mRNA level of Nrf2 and protein levels of Nrf2 and heme oxygenase-1(P＜0.05,P＜0.01),lowered levels of tumor necrosis factor-α and interleukin-6,and down-regulated mRNA levels of Keap1 and NF-κB p65 and protein levels of Keap1,NF-κB p65,phospho-inhibitory subunit of NF-κB α in the liver(P＜0.05,P＜0.01).Conclusion:Jinzhao Capsules can protect mice from chronic alcoholic liver injury by enhancing the activities of alcohol metabolism-related enzymes and antioxidant enzymes in the liver tissue and regulating the levels of inflammatory mediators and the mRNA and protein levels of factors in the Keap1/Nrf2/NF-κB pathway.