世界中医药2024，Vol.19Issue(12)：1802-1810,9.DOI:10.3969/j.issn.1673-7202.2024.12.015
结直肠癌患者舌苔变化与舌苔菌群和肠道菌群的相关性研究
Correlations of Tongue Coating Changes with Tongue Coating Microbiota and Gut Microbiota in Patients with Colorectal Cancer
摘要
Abstract
Objective:To explore the correlations of tongue coating changes with and tongue coating microbiota and gut microbiota in the patients with greasy tongue coating and colorectal cancer.Methods:A total of 63 patients with colorectal cancer treated in the Department of Colorectal Surgery of the First Affiliated Hospital of Harbin Medical University from September 2022 to March 2023 were enrolled in this study.Based on the characteristics of tongue coatings,12 patients with yellow greasy tongue coating and 9 pa-tients with white greasy tongue coating were selected by stratified random sampling,and the tongue coating,cancer tissue,and adja-cent tissue were sampled from these patients.Meanwhile,tongue coating samples were collected from 6 healthy volunteers with thin white tongue coating and no abnormalities during colonoscopy as the control group.All the samples were subjected to high-through-put 5R 16S rDNA sequencing.Results:The patients with colorectal cancer mainly presented yellow greasy tongue coating and white greasy tongue coating.Atopobium in the tongue coating and Granulicatella in the gut might be characteristic bacteria in the patients with yellow greasy tongue coating.Gemella in the tongue coating and Roseburia and Shewanella in the gut might be characteristic bacterial genera in the patients with white greasy tongue coating.Fusobacterium nucleatum,Actinomyces odontolyticus,and Pep-tostreptococcus stomatis might be potential oral and intestinal bacteria in the colorectal cancer patients with yellow greasy tongue coating and white greasy tongue coating,respectively.Conclusion:The changes of tongue coating in colorectal cancer patients are related to the changes of the structure and abundance of gut microbiota and tongue coating microbiota.The different overlapping species and degrees between tongue coating microbiota,cancer tissue microbiota,and adjacent tissue microbiota suggest that the changes in greasy tongue coating in traditional Chinese medicine can reflect the characteristic changes in the oral and intestinal mi-crobiota in the patients with colorectal cancer.The findings provide a theoretical basis for the later research on the Chinese medi-cines for colorectal cancer by targeting microorganisms.关键词
结直肠癌/舌苔/舌诊/舌苔菌群/肠道菌群/5R 16S rDNA测序/相关性Key words
Colorectal cancer/Tongue coating/Tongue diagnosis/Tongue coating microbiota/Gut microbiota/5R 16S rDNA se-quencing/Correlation分类
中医学引用本文复制引用
屈德春,吕琨,朱跃坤,吴海艳,刘雪丽,徐巍..结直肠癌患者舌苔变化与舌苔菌群和肠道菌群的相关性研究[J].世界中医药,2024,19(12):1802-1810,9.基金项目
国家区域中医(专科)诊疗中心(肿瘤)培育单位项目(国中医药医政涵[2018]205号) （专科）
国家中医优势专科建设项目(黑中医药综涵[2022]36号) （黑中医药综涵[2022]36号）
黑龙江省中医药科研项目(ZHY2020-166) （ZHY2020-166）
哈尔滨医科大学附属第一医院科研创新基金项目(2020M25) （2020M25）
