色谱2024，Vol.42Issue(8)：731-739,9.DOI:10.3724/SP.J.1123.2024.01014
超高效液相色谱-三重四极杆质谱法同时检测植物油中21种全氟及多氟烷基化合物
Simultaneous determination of 21 perfluorinated and polyfluoroalkyl substances in plant oils by ultra-high performance liquid chromatography-triple quadrupole mass spectrometry
摘要
Abstract
Edible plant oils are a key component of the daily human diet,and the quality and safety of plant oils are related to human health.Perfluorinated and polyfluoroalkyl substances(PFASs)are pollutants that can contaminate plant oil through the processing of raw materials or exposure to materials containing these substances.Thus,establishing a sensitive and accu-rate analytical method for the determination of PFASs is critical for ensuring the safety of plant oils.In this study,a method based on acetonitrile extraction and solid phase extraction purifi-cation combined with ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UHPLC-MS/MS)was developed for the simultaneous determination of 21 PFASs,including perfluorocarboxylic acids,perfluoroalkyl sulfonic acids,and fluorotelomer sulfonic acids,in edible plant oils.The chromatographic conditions and MS parameters were optimized,and the influences of the extraction solvents and purification method were systemat-ically studied.Plant oil samples were directly extracted with acetonitrile and purified using a weak anion-exchange(WAX)column.The 21 target PFASs were separated on a reversed-phase C18 chromatographic column and detected using a triple quadrupole mass spectrometer with an electrospray ionization source.The mass spectrometer was operated in negative-ion mode.The target compounds were analyzed in multiple reaction monitoring(MRM)mode and quantified using an internal standard method.The results demonstrated that the severe interference ob-served during the detection of PFASs in the co-extracted substances was completely eliminated after the extraction mixture was purified using a WAX column.The 21 target PFASs showed good linearity in their corresponding ranges,with correlation coefficients greater than 0.995.The limits of detection(LODs)and limits of quantification(LOQs)of the method were in the range of 0.004-0.015 and 0.015-0.050 μg/kg,respectively.The recoveries ranged from 95.6％to 115.8％,with relative standard deviations(RSDs)in the range of 0.3％-10.9％(n=9).The established method is characterized by simple sample pretreatment,good sensitivity,high im-munity to interferences,and good stability,rendering it suitable for the rapid analysis and accu-rate determination of typical PFASs in edible plant oils.关键词
超高效液相色谱-三重四极杆质谱/固相萃取/全氟及多氟烷基化合物/植物油Key words
ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UHPLC-MS/MS)/solid phase extraction(SPE)/perfluorinated and polyfluoroalkyl sub-stances(PFASs)/plant oil分类
化学化工引用本文复制引用
冯灏,张炜,何保山,李盼盼,高树青,郭宝元,杨永坛..超高效液相色谱-三重四极杆质谱法同时检测植物油中21种全氟及多氟烷基化合物[J].色谱,2024,42(8):731-739,9.基金项目
中央级公益性基本科研业务费专项资金项目(ZX2241).Fundamental Research Funds of Nonprofit Central Institutes(No.ZX2241). （ZX2241）
