色谱2024，Vol.42Issue(8)：805-811,7.DOI:10.3724/SP.J.1123.2023.11008
离子色谱指纹图谱分析在烟用香精香料品质评价中的应用
Application of ion chromatographic fingerprint analysis for quality evaluation of tobacco flavors
摘要
Abstract
Tobacco flavor,an important tobacco additive,is an essential raw material in ciga-rette production that can effectively improve the quality of tobacco products,add aroma and taste,and increase the suction flavor.The quality consistency of tobacco flavors affects the quali-ty stability of branded cigarettes.Therefore,the quality control of tobacco flavors is a major con-cern for cigarette and flavor manufacturers.Physical and chemical indices,odor similarity,and sensory efficacy are employed to evaluate the quality of tobacco flavors,and the analysis of chemical components in tobacco flavors is usually conducted using gas chromatography(GC)and high performance liquid chromatography(HPLC).However,because the composition of tobacco flavors is complex,their quality cannot be fully reflected using a single component or combination of components.Therefore,establishing an objective analytical method for the quality control of tobacco flavors is of extreme importance.Chromatographic fingerprint analysis is rou-tinely used for the discriminative analysis of tobacco flavors.Chromatographic fingerprints refer to the general characteristics of the concentration profiles of different chemical compounds.In the daily procurement process,fingerprints established by GC and HPLC are effective for the evaluation and identification of tobacco flavors.However,given continuous improvements in aro-ma-imitation technology,some flavors with high similarity cannot be directly distinguished using existing methods. In this study,a method for the determination of organic acids and inorganic anions in tobacco flavors based on ion chromatography(IC)was developed to ensure the quality consistency of to-bacco flavors.A 1.0 g sample of tobacco flavors and 10 mL of deionized water were mixed and vi-brated for 30 min.The aqueous sample solution was passed through a 0.45 μm membrane filter and RP pretreatment column in succession to eliminate interferences and then subjected to IC.Standard solutions containing nine organic acids and seven inorganic anions were used to identify the anions in the tobacco flavors,and satisfactory reproducibility was obtained.The relative standard deviations(RSDs)for retention times and peak areas were＜0.71％and＜6.02％,re-spectively.The chromatographic fingerprints of four types of tobacco flavors(samples A-D)from five different batches were obtained.Nine tobacco flavor samples from different manufactur-ers(samples AY1-AY3,BY1-BY2,CY1-CY2,DY1-DY2)were also analyzed to obtain their chro-matographic fingerprints.Hierarchical cluster and similarity analyses were used to evaluate the quality of tobacco flavors from different manufacturers.Hierarchical clustering refers to the process of subdividing a group of samples into clusters that exhibit a high degree of intracluster similarity and intercluster dissimilarity.The dendrograms obtained using SPSS 12.0 indicated good quality consistency among the samples in different batches.Samples AY3,BY2,CY2,and DY1 clustered with the batches of standard tobacco flavors.Therefore,hierarchical cluster analy-sis can effectively distinguish the quality of products from different manufacturers.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(version 2.0)was used to evaluate the similarity between the standard tobacco flavors and products from different manufacturers.Among the samples analyzed,samples AY3,BY2,CY2,and DY1 showed the highest similarity values(＞97.7％),which was consistent with the results of the hierarchical cluster analysis.This finding indicates that IC combined with chromatographic fingerprint analysis could accurately determine the quality of tobacco flavors.GC combined with ultrasonic-assisted liquid-liquid extraction was also used to analyze the tobacco flavors and verify the accuracy of the proposed method.Compared with GC coupled with ultrasonic-assisted liquid-liquid extraction,IC demonstrated more significant quality differences among certain tobacco flavors.关键词
离子色谱法/烟用香精香料/色谱指纹图谱/聚类分析/相似度分析Key words
ion chromatography(IC)/tobacco flavors/chromatographic fingerprint/hierar-chical cluster analysis/similarity analysis分类
化学化工引用本文复制引用
许高燕,施宏,何歆滢,张丽娜,刘崇盛,高阳,黄忠平,刘会君,吴兆明,章瑞潮..离子色谱指纹图谱分析在烟用香精香料品质评价中的应用[J].色谱,2024,42(8):805-811,7.基金项目
浙江中烟工业有限责任公司科技项目(ZJZY2022C001).Science and Technology Program of China Tobacco Zhejiang Industry Co.,Ltd.(No.ZJZY2022C001). （ZJZY2022C001）
