水生生物学报2024，Vol.48Issue(8)：1322-1332,11.DOI:10.7541/2024.2024.0057
ZnO/PDMS对生物被膜形成和厚壳贻贝稚贝附着的影响及在养殖网箱的潜在应用
ZNO/PDMS ON MARINE BIOFILM FORMATION AND ATTACHMENT OF MYTILUS CORUSCUS AND THEIR POTENTIAL APPILICATIONS IN AQUACULTURE CAGES
摘要
Abstract
Cage aquaculture plays an essential role in marine aquaculture.However,the negative impact of biofouling shortens the cleaning cycle of the cage,leading to increased maintenance cost.Additionally,biofouling obstructs oxygen supply and waste removal from the cage,heightening the risk of infection among aquatic organisms.This issue poses a significant challenge to the advancement of aquaculture.Currently,methods to combat marine biofouling mostly remain in the laboratory stage because of the tedious production process and lack of practical verification.Therefore,it is meaningful to develop an economical and effective antifouling coating for aquaculture cages.Biofilm formed by bacteria and diatoms facilitate the settlement of marine invertebrates such as Mytilus coruscus,which is the key to solve the problem of marine biofouling.Polydimethylsiloxane(PDMS)is a typical fouling-release coating known for its viscoelasticity and hydrophobicity.The lack of mechanical strength and the performance of long-term confrontation with diatom adhesion uncovered the limitations of PDMS.The conventional solution is to incorporate other materials into PDMS,which can improve the mechanical properties of PDMS and allow PDMS to inhibit the microfouling formed by bacteria and diatoms.Nano-zinc oxide particles(ZnO NPs)are characterized by small particle size and high specific surface area,showing excellent antibacterial properties,which can be combined with PDMS as a bacteriostat.In this study,ZnO/PDMS coating made from PDMS filled with different concentrations of ZnO NPs was used to improve the antifouling ability of cages.The effects of Glass,PDMS,and ZnO/PDMS(ZnO NPs concentra-tions of 3.5wt％,7.5wt％,11.25wt％,and 15wt％)on natural biofilms and subsequent mussel settlement were tested in field bioassay.Compared with Glass and PDMS,the settlement rate of M.coruscus decreased by 75.41％and 62.50％,respectively,on 15wt％ZnO/PDMS-coated surfaces with 28-day-old biofilms.Furthermore,at 28 days of age,the bacterial density of the biofilm formed on 15wt％ZnO/PDMS was reduced by 43.25％and 40.44％,respectively,compared to Glass and PDMS.The diatom density decreased by 91.59％and 13.32％,respectively,compared with Glass and PDMS in 28-day-old biofilm.MiSeq sequencing confirmed differences in bacterial communities among substrates.Representatively,the relative abundance of Psychrobacter decreased,while the relative abundance of Wino-gradskyella increased on 15wt％ZnO/PDMS.Structural Equation Model results indicate that ZnO/PDMS could inhibit M.coruscus attachment by reducing natural biofilm biomass and changing the bacterial community.This study fills the gap in the effect of ZnO/PDMS coating on the bacterial community of natural biofilms and provides a novel solution for the biofouling in cage aquaculture.关键词
ZnO/PDMS/生物被膜/厚壳贻贝/养殖网箱/细菌群落Key words
ZnO/PDMS/Biofilm/Mytilus coruscus/Cage aquaculture/Bacterial community分类
生物科学引用本文复制引用
李局,李政,杨金龙,梁箫..ZnO/PDMS对生物被膜形成和厚壳贻贝稚贝附着的影响及在养殖网箱的潜在应用[J].水生生物学报,2024,48(8):1322-1332,11.基金项目
国家重点研发计划(2023YFE0115500、2023YFD2401902和22022YFE0204600)资助[Supported by the National Key Research and Development Program of China(2023YFE0115500,2023YFD2401902 and 22022YFE0204600)] （2023YFE0115500、2023YFD2401902和22022YFE0204600）
