水生生物学报2024，Vol.48Issue(8)：1333-1347,15.DOI:10.7541/2024.2023.0405
绿水螅野生型品系及无藻品系对聚苯乙烯纳米颗粒胁迫差异化响应的转录组分析
TRANSCRIPTOMIC ANALYSIS OF DIFFERENTIATED RESPONSES BETWEEN SYMBIOTIC AND APOSYMBIOTIC STRAIN OF HYDRA SINENSIS TO POLYSTYRENE NANOPARTICLES
摘要
Abstract
To investigate the impact of symbiotic algae presence or absence on the tolerance of Hydra sinensis to polystyrene nanoparticles(PS NPs),we conducted a 48h acute toxicity experiment on both symbiotic and aposymbiotic strain of H.sinensis using different PS NPs concentrations(20 nm in diameter).The results showed that the 48h half-lethal concentration(48h LC50)for the symbiotic strain(3.36×102 mg/L)was significantly higher than that of the aposymbiotic strain(1.39×102 mg/L).Subsequently,following exposure to a medium containing 75 mg/L PS NPs for 48h,both symbiotic and aposymbiotic strains underwent transcriptomic analysis,respectively(with a control group having 0 PS NPs).A total of 1532 differentially expressed genes(DEGs)were identified in the symbiotic strain between the PS NPs-treated and control groups,with 763 were up-regulated and 769 down-regulated genes.Similarly,1079 differentially expressed genes(DEGs)were obtained between PS NPs-treated and control group of the symbiotic strain,with 476 up-regulated and 603 down-regulated genes.KEGG enrichment analysis revealed significant enrich-ment(P＜0.05)of metabolic pathways in both strains,encompassing immune response,pathological indications,mate-rial metabolism,cellular processes,organic systems,environmental information processing,and genetic information processing.Among the pathways related to pathological indications,all DEGs were upregulated in only one out of 21 significant pathways for the symbiotic strain,while for the aposymbiotic strain,all DEGs were upregulated in 9 out of 14 significant pathways.This suggests a significantly higher activation level of pathological indicators in the aposymbio-tic strain under PS NPs stress compared to the symbiotic strain,indicating a superior tolerance at the molecular level.In addition,all DEGs related to immune response in the aposymbiotic strain treated with PS NPs were upregulated,while only approximately half of the DEGs in the symbiotic strain were upregulated.This suggests a lower immune response sensibility to PS NPs in the symbiotic strain compared to the aposymbiotic strain.In summary,the difference in immune response sensibility to PS NPs between the two H.sinensis strains may underlie their differing tolerance to PS NPs stress at the molecular level.In addition,the symbiotic strain exhibits lower immune response sensitivity to PS NPs,possibly due to the host downregulating immune levels against foreign invaders to accommodate symbiotic algae.The study provides basic data for revealing the biotoxicity and molecular mechanism of nano-sized plastic particles.关键词
聚苯乙烯纳米颗粒/急性毒理实验/转录组/免疫应答/病态指征/中国绿水螅Key words
Polystyrene nanoparticles/Acute toxicity test/Transcriptome/Immune response/Pathological indication/Hydra sinensis分类
生物科学引用本文复制引用
杨凯宁,符乐一,李桂丽,卜莉萍,潘红春..绿水螅野生型品系及无藻品系对聚苯乙烯纳米颗粒胁迫差异化响应的转录组分析[J].水生生物学报,2024,48(8):1333-1347,15.基金项目
国家自然科学基金(42077208和31671529)资助[Supported by the National Natural Science Foundation of China(42077208 and 31671529)] （42077208和31671529）
