水生生物学报2024，Vol.48Issue(8)：1365-1377,13.DOI:10.7541/2024.2024.0018
黄颡鱼XX伪雄鱼制备与全雌种群规模化繁育
INDUCTION OF XX GENOTYPIC PSEUDO-MALES AND LARGE-SCALE REPRODUCTION OF ALL-FEMALE POPULATION IN YELLOW CATFISH(TACHYSURUS FULVIDRACO)
摘要
Abstract
There is a critical shortage of female broodstock in the yellow catfish industry,making the production of all-female populations using sex-control technology extremely important to meet the demand for seedling production and genetic improvement.In this study,XX genotypic yellow catfish fry were treated with 17α-methyl dihydrotestosterone(MDHT)and Letrozole(LZ)through feed during the critical period of sex differentiation(12 to 60 days post-hatching,DPH).Five experimental groups were established:MH(50 mg/kg MDHT),ML(10 mg/kg MDHT),MH+L(50 mg/kg MDHT,1000 mg/kg LZ),ML+L(10 mg/kg MDHT,1000 mg/kg LZ),and control group(CR,0 MDHT,0 LZ).The ML and ML+L groups obtained the highest proportions of masculinization,at 66.7％and 83.3％,respectively.Sex-reversed individuals in these groups included complete-testis-type(29.2％,33.3％)and intersex gonads(37.5％,50.0％),both of which produced 43.8％functional testes.Using XX pseudo-males as fathers and selecting XX females as mo-thers for multiple generations,2800000 XX genotypic fry were obtained through induced spawning.In an outdoor earthen pond culture environment,it was found that the ovaries of 98.0％of individuals developed well,while 2.0％of individuals reversed to phenotypic males at 92 DPH.At this point,our research team has established a large-scale tech-nological system for producing all-female yellow catfish populations,providing crucial support for large-scale induced spawning and genetic improvement,and serving as an important guarantee for the healthy development of the yellow catfish industry.关键词
性别控制/加氢甲基睾酮/来曲唑/全雌种群/雄性化/黄颡鱼Key words
Sex control/17α-methyldihydrotestosterone/Letrozole/All-female/Masculinization/Tachysurus ful-vidraco分类
农业科技引用本文复制引用
丁洋,樊启学,牟长军,沈志刚,鲁子怡,刘娅,曹霄,王亚,梁浩文,任旌雷,邓翔,何焱..黄颡鱼XX伪雄鱼制备与全雌种群规模化繁育[J].水生生物学报,2024,48(8):1365-1377,13.基金项目
国家自然科学基金面上项目(32273122) （32273122）
科技创新2030-重大项目(2023ZD04068)资助[Supported by the National Natural Science Foundation of China(32273122) （2023ZD04068）
Technology Innovation 2030 Major Project(2023ZD04068)] （2023ZD04068）
