水生生物学报2024，Vol.48Issue(8)：1393-1401,9.DOI:10.7541/2024.2024.0048
聚球藻PCC 7002 phoR基因的鉴定及其在磷稳态中的功能
IDENTIFICATION AND FUNCTION OF PHOR GENE INVOLVED IN PHOSPHO-RUS HOMEOSTASIS IN SYNECHOCOCCUS SP.PCC 7002
摘要
Abstract
To explore the mechanism of phosphorus limitation adaptation in cyanobacteria,a possible phosphorus regu-latory gene,phoR(A2100),was identified in Synechococcus sp.PCC 7002.The results of bioinformatics analysis showed that the length of phoR was 1296 bp,encoding 431 amino acids.PhoR comprises a PAS domain,a HisKA domain and a HATPase_c domain,with a theoretical isoelectric point of 5.20 and a predicted molecular weight of 49.58 kD.In addition,the sequence of PhoR is relatively conserved in different cyanobacteria species.Subsequently,the PhoR recombinant protein was expressed in E.coli,and a murine polyclonal antibody was prepared to detect the expression in Synechococcus sp.PCC 7002 under the phosphorus deprivation induction.The results indicated that the expression of PhoR was significantly induced in response to P deficiency.Then,we generated the Mut-phoR mutant strain through deletion mutagenesis and analyzed its physiological phenotype.It was found that Mut-phoR exhibited notable growth defects and impaired photosynthesis under phosphate limitation.This study provides a scientific basis for further under-standing on the molecular mechanism of phosphate deficiency adaptation in cyanobacteria.关键词
phoR/原核表达/基因敲除/多克隆抗体制备/聚球藻PCC 7002Key words
phoR/Prokaryotic expression/Gene knockout/Polyclonal antibody preparation/Synechococcus sp.PCC 7002分类
生物科学引用本文复制引用
孙巧伟,冯苗苗,陈思宁,陈未中,姜海波..聚球藻PCC 7002 phoR基因的鉴定及其在磷稳态中的功能[J].水生生物学报,2024,48(8):1393-1401,9.基金项目
国家自然科学基金(42188102、32170108和91951111) （42188102、32170108和91951111）
宁波市重点研发计划(2022Z189) （2022Z189）
宁波市公益基金重点项目(2023S040) （2023S040）
南方海洋科学与工程广东省实验室(珠海)自主科研项目(SML2021SP204)资助[Supported by the National Natural Science Foundation of China(42188102,32170108 and 91951111) （珠海）
Ningbo Key R & D Program(2022Z189) （2022Z189）
Ningbo Public Welfare Science and Technology Program(2023S040) （2023S040）
Independent Research Projects of Southern Marine Science and Engineer-ing Guangdong Laboratory(SML2021SP204)] （SML2021SP204）
