生物加工过程2024，Vol.22Issue(4)：378-387,10.DOI:10.3969/j.issn.1672-3678.2024.04.003
双菌共培养产氢体系的构建及产氢性能的优化
Construction and performance of hydrogen production system bythe co-culture of Clostridium butyricum and Enterobacter aerogenes
摘要
Abstract
Bio-hydrogen production is a technology to convert the chemical energy stored in organics to hydrogen by the efficient hydrogen producing bacteria in nature.Based on the previous studies on the optimization of hydrogen production conditions for single hydrogen producing bacteria,a co-culture system for hydrogen production was constructed using Clostridium butyricum LQ25 and Enterobacter aerogenes AJ110637,and the corresponding hydrogen production performance and synergistic effect were subsequently evaluated with glucose as the substrate.As a result,the co-culture system of C.butyricum LQ25 and E.aerogenes AJ110637 could provide the highest hydrogen production efficiency under the conditions with the inoculum ratio of 9∶1,initial pH 7.0,and fermentation temperature of 40℃,with a maximum hydrogen production of 354.05 mL H2/g glucose,which was 31.71%and 70.59%higher than C.butyricum and E.aerogenes systems,respectively.Notably,the addition of 1.0 g/L calcium carbonate could significantly increase the hydrogen yield by 21.80%compared with the control group,likely through alleviating the acidity of the co-culture system.Overall,the hydrogen production rate in the co-culture system of C.butyricum and E.aerogenes was higher than either single system,reflecting the synergistic effect of C.butyricum and E.aerogenes to a certain extent.关键词
丁酸梭菌/产气肠杆菌/产氢/发酵/共培养Key words
Clostridium butyricum/Enterobacter aerogenes/hydrogen/fermentation/co-cultivation分类
生物科学引用本文复制引用
张新,王建米,蒋永琪,付欢,周俊,吴夏芫,雍晓雨,贾红华..双菌共培养产氢体系的构建及产氢性能的优化[J].生物加工过程,2024,22(4):378-387,10.基金项目
国家重点研发计划项目(2018YFA0902200) （2018YFA0902200）
江苏省创新支撑计划国际科技合作/港澳台合作(港澳台科技合作项目,BZ2022052) （港澳台科技合作项目,BZ2022052）
