黄芩苷调控氧化应激治疗糖尿病肾病OACSTPCD

This work was to explore the therapeutic effects of baicalin on mice with diabetic nephropathy and its possible mechanism.40 C57BL/6 mice were randomly divided into 5 groups,with 8 mice in each group.Except the control group,high fat diet and STZ were used to establish the mice model with diabetic nephropathy.Baicalin was administered at 100,200 and 400 mg/kg/day in low,medium and high dose groups,respectively.Meanwhile,the control and model groups were dosed with the same amount of saline.After 16 weeks,fasting blood glucose,weight,24 h urine volume and urinary albumin excretion rate(UAE)were detected,and the contents of Malondialdehyde(MDA),Catalase(CAT),blood urea nitrogen(BUN)and serum creatinine(Scr)were measured by automatic biochemical analyzer;the tissues were stained by HE,PAS and Masson.In addition,the expression of 8-OHdG and FOXO3a were analyzed by immunohistochemistry,and the expression of CAT m RNA and Mn-SOD m RNA was detected by real-time quantitative PCR,the expression of FOXO3a protein in reval tissues was detected by western blotting.As a result,the treatment with baicalin led to the decrease of the fasting blood glucose,renal index,MDA content,BUN and Scr,24 h urine and UAE,while the CAT contents increased(P＜0.01,P＜0.05).Based on the staining experiments,the proliferation and fibrosis of Mesangial cells were improved in baicalin groups,as well as the hyperemia and edema.Moreover,the infiltration of inflammatory cells decreased after baicalin treatment.The decrease of 8-OHdG expression and increase of FOXO3a expression were observed,along with the higher levels for m RNA expression of CAT and Mn-SOD(P＜0.05).Western blotting analysis showed higher expression of FOXO3a protein compared with the model group.Overall,baicalin could effectively reduce blood sugar,alleviate renal tissue injury,improve renal function,prevent and treat diabetic nephropathy.Regarding its mechanism of action,the treatment with baicalin could activate the FOXO3a signaling,leading to the reduction of MDA and 8-OHdG expression,along with higher CAT and Mn-SOD expression.The scavenging of oxygen free radicals could maintain the balance of oxidative stress levels in the body.