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黑水虻酶解液美拉德反应及其风味化合物分析OACSTPCD

Maillard reaction of enzymatic hydrolysate from black soldier flies and analysis of flavor compounds

中文摘要英文摘要

为了开发新的宠物诱食剂,以黑水虻幼虫为原料,对酶解黑水虻幼虫后的酶解液在不同温度下进行美拉德反应,通过测定类黑精含量和小分子中间产物含量来考察反应温度对美拉德反应的影响,使用气相色谱质谱(GC-MS)结合主成分分析法对美拉德反应产物的风味化合物进行分析,探讨不同温度下美拉德反应产物的差异及特征成分.结果表明:在设定的 90~130℃条件下,温度越高,美拉德反应的褐变程度越高;当温度为 110℃时,美拉德反应产物中风味化合物的总含量最高,为2.373 μg/g.通过主成分分析可知,在110℃时美拉德反应产生主要特征化合物最多,分别为甲酸乙酯、乙酸、庚醛、仲辛酮、苯甲醇、丁酸、2-庚酮、正辛醇、苯酚、己醇、己醛和壬酸.可见,在美拉德反应温度为 110℃时,得到的产物具有最佳风味.

The development of new animal food attractants has become a hot research topic in recent years.Herein,we report Maillard reaction of the enzymatic hydrolyzed solution from black soldier fly larvae at different temperatures,as well as the analysis of resultant flavor compounds by GC-MS.As a result,higher Browning degree of Maillard reaction was observed at elevated temperature under the conditions of 90-130℃range.When Maillard reaction was conducted at 110℃,the total content of flavor products reached the highest level of 2.373 μg/g,and the major components were characterized by GC-MS as the following:ethyl formate,acetic acid,heptanal,sec-octanone,benzyl alcohol,butyric acid,2-heptanone,n-octanol,phenol,hexanol,hexanal,nonanoic acid.Based on the above results,the optimal temperature for Maillard reaction was 110℃,from which the best flavor and sensory quality could be achieved for the product.

汪雨晗;朱本伟;余养朝;冯占;李贺文;张靖;李新福;熊强

南京工业大学 食品与轻工学院,江苏 南京 211800江苏华绿生物科技股份有限公司,江苏 宿迁 223700

生物工程

黑水虻美拉德反应酶解液风味物质宠物食品

black soldier flyMaillard reactionenzymatic hydrolyzateflavor substancespet food

《生物加工过程》 2024 (004)

426-431 / 6

江苏省重点研发计划(现代农业)(BE2019372)

10.3969/j.issn.1672-3678.2024.04.009

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