生物技术通报2024，Vol.40Issue(7)：55-67,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0033
植物DNA双链断裂修复机制及其在重离子诱变和基因编辑中的作用
Repair Mechanisms of DNA Double-strand Breaks and Their Roles in Heavy Ion Mutagenesis and Gene Editing in Plants
摘要
Abstract
Plants are often subjected to DNA damage due to a range of environmental and internal factors in the natural world.Among these factors,DNA double strand breaks(DSBs)have the most serious consequences to plants.If these DSBs are repaired improperly,which may result in genome instability,gene mutations,and even cell death.On one hand,plants have evolved a powerful and well-organized damage repair mechanism to ensure overall survival and normal reproduction.On the other hand,based on the fault-tolerance and mutagenicity of the repair process,a series of techniques such as T-DNA insertion,gene editing,and physical mutagenesis have been widely used for variety improvement in plants and animals.Compared with mammals,reports on DSBs repair pathways and their molecular mechanisms in plants are limited.In this paper,we review the responses of plants to DSBs damage and the main repair mechanisms,and we also focus on the recent advances in alternative end joining(Alt-EJ),whose mechanism has not yet been fully revealed.In addition,we discuss the characteristics and multi-pathway options of DSBs repair in plants induced by heavy ion beams irradiation,and the progress of gene editing technology based on different DSBs repair pathways.This study aims to provide reference for understanding the molecular mechanism of plant DSBs damage response and developing efficient biological breeding technology.关键词
植物DSBs损伤修复/同源重组/非同源末端连接/重离子束/基因编辑Key words
damage repair of plant DSBs/homologous recombination/non-homologous end joining/heavy ion beams/gene editing引用本文复制引用
隆静,陈婧敏,刘霄,张一凡,周利斌,杜艳..植物DNA双链断裂修复机制及其在重离子诱变和基因编辑中的作用[J].生物技术通报,2024,40(7):55-67,13.基金项目
国家自然科学基金项目(12135016,11975285),西南作物基因资源发掘与利用国家重点实验室开放课题(SKL-KF202225),国家重点研发计划(2022YFD1200705),广东省重点领域研发计划项目(2022B0202060006),核能开发科研项目(E11Z211GQ1) （12135016,11975285）
