生物技术通报2024，Vol.40Issue(7)：163-171,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0162
甜瓜BZR转录因子家族基因的全基因组鉴定及表达模式分析
Genome-wide Identification and Expression Pattern Analysis of BZR Transcription Factor Gene Family of Melon
摘要
Abstract
[Objective]Brassinazole-resistant(BZR)is a plant specific transcription factor that plays a crucial role in plant growth and development.Identifying members of the CmBZR gene family across the whole melon genome,and analyzing the expression patterns of related gene,may provide a basis for further exploring the biological functions of the melon BZR gene family.[Method]Based on the whole melon genome data,the BZR family genes in melon were identified using BLAST.Bioinformatics methods were used to analyze the physicochemical properties,chromosomal distribution,gene structure,protein domains,phylogenetic evolution,and cis acting elements of the family proteins.Fluorescence quantitative PCR was used to verify the expression of BZR family genes in different tissues and fruits of melon at different growth and development stages.[Result]A total of 6 BZR genes were identified in cantaloupe,which can be divided into 5 subfamilies through phylogenetic analysis.CmBZR genes are distributed on chromosomes 3,7,8,11,and 12.All BZR proteins have conserved domains.The promoter region of the CmBZR gene is significantly enriched with cis-acting elements related to growth and development,hormone signal transduction,and abiotic stress.CmBEH1-4 is highly expressed in stems,female flowers,and ovaries,and is also expressed in growth,maturity,respiratory climacteric,and late respiratory climacteric stages.[Conclusion]Six members of the melon CmBZR gene family have been systematically identified across the whole genome,and different genes are expressed in various tissues and growth stages of the melon,and their expression patterns are various.关键词
甜瓜/BZR/转录因子/全基因组鉴定/表达分析/生物信息学Key words
melon(Cucumis melo L.)/BZR/transcription factor/genome-wide identification/expression analysis/bioinformatics引用本文复制引用
臧文蕊,马明,砗根,哈斯阿古拉..甜瓜BZR转录因子家族基因的全基因组鉴定及表达模式分析[J].生物技术通报,2024,40(7):163-171,9.基金项目
内蒙古大学国家级大学生创新创业项目(202310126034) （202310126034）
