生物技术通报2024，Vol.40Issue(7)：172-182,11.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0094
藜麦FLS基因家族的鉴定、表达及DNA变异分析
Identification,Expression and DNA Variation Analysis of FLS Gene Family in Chenopodium quinoa
摘要
Abstract
[Objective]This study is to identify and analyze the flavonol synthetase FLS gene family as a key enzyme in the secondary metabolism of polyphenols in Caryophyllum plants and to explore the function of FLSs in Chenopodium quinoa.[Method]Bioinformatics analysis website was used to identify the CqFLS family members,and analyze their gene structure,protein physicochemical properties,secondary structure,tertiary structure,promoter cis element and phylogenetic relationship.The gene cloning and expression vector construction was to analyze their protein expression.[Result]The 3 CqFLSs genes were identified,and they were unevenly distributed on two chromosomes,and the promoter region of CqFLSs contained salicylic acid,abscisic acid,methyl jasmonate and drought-induced elements.There were multiple InDel and SNP variations in CqFLS2.1g,the coding nucleotides were deleted at ch01 28871893,28871125,and 28872881,and all of them were annotated as upstream effects,and no frameshift mutations were detected.Phylogenetic tree analysis showed that CqFLS2.1g were in different branches from CqFLS1.1g and CqFLS3.10g,their expressions varied,and CqFLS2.1g were also differentiated.Meanwhile gene expression analysis revealed that the overall expressions of the three CqFLSs genes in"Qingbai 1"grains were higher than those of"Qinghei 1"and"Gongzha 4".The cloned CqFLS1.1g was induced with 0.3 mmol/L IPTG and was successfully expressed at both 20℃and 37℃.[Conclusion]CqFLS1.1g plays a role in the formation of flowers and grain development,while CqFLS2.1g is mainly involved in the formation of quinoa grains,and the expression of CqFLS is tissue-specific and plays an important role in the growth and development of quinoa.关键词
藜麦/黄酮醇合酶/荧光定量PCR/DNA变异/原核表达Key words
Chenopodium quinoa/flavonol synthase/qRT-PCR/DNA variation/prokaryotic expression引用本文复制引用
孙慧琼,张春来,王锡亮,徐宏申,窦苗苗,杨博慧,柴文婷,赵珊珊,姜晓东..藜麦FLS基因家族的鉴定、表达及DNA变异分析[J].生物技术通报,2024,40(7):172-182,11.基金项目
黄土高原特色作物优质高效生产省部共建协同创新中心基金项目(SBGJXTZXKF-11),国家自然科学基金项目(31971994),山西农大生物育种工程项目(YZGC104),山西省重点研发计划项目(201803D221012-2),中国科技部中巴国际合作项目(KY202002002),山西农谷建设专项(SXNGJSKYZX201702,SXNGJSKYZX201704) （SBGJXTZXKF-11）
