生物技术通报2024，Vol.40Issue(7)：226-234,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0160
枯草芽孢杆菌B579对黄瓜枯萎病的防治及其诱导抗性研究
Study on the Control and Induced Resistance in Cucumber with Bacillus subtilis B579 against Cucumber Fusarium Wilt
摘要
Abstract
[Objective]To explore the control effect and mechanism of Bacillus subtilis B579 on cucumber Fusarium wilt.[Method]Plate confrontation test and greenhouse pot experiment were used to detect the growth and morphological changes of the pathogen,plant growth,activity of defense enzymes in leaves and malondialdehyde content,and expression of defense-related related genes.[Result]The B.subtilis B579 had a strong inhibitory effect on cucumber Fusarium wilt,and the mycelium around the inhibition zone showed uneven thickness,swelling and breakage.Pot experiment demonstrated that B579 achieved the disease control efficiency against cucumber wilt,reaching 78.80%.The plant height,stem diameter,fresh weight,and dry weight of cucumber seedlings in B579 treatment increased by 13.87%,4.17%,15.15%and 12.77%compared with the control group,respectively.After pretreatment with B579,pathogen inoculation significantly increased the activities of phenylalanine ammonilyase(PAL),polyphenol oxidase(PPO),peroxidase(POD),and catalase(CAT)and reduced the content of malondialdehyde(MDA)in cucumber leaves on day 4 and 7 compared with the control group.The results of real-time fluorescence quantitative PCR showed that the expressions of LOX,PR1,PR3 and CAT genes in cucumber leaves after inoculation with B579 and then challenge with pathogen were significantly higher than those in other treatments at 4 and 7 d.[Conclusion]B.subtilis B579 can control cucumber Fusarium wilt by directly inhibiting the growth of pathogen and inducing resistance in cucumber.关键词
枯草芽孢杆菌B579/促生/黄瓜枯萎病/诱导抗性/生物防治Key words
Bacillus subtilis B579/promoting growth/cucumber Fusarium wilt/induced resistance/biological control引用本文复制引用
范宗强,冯靖涵,郑丽雪,王硕,彭向前,陈芳..枯草芽孢杆菌B579对黄瓜枯萎病的防治及其诱导抗性研究[J].生物技术通报,2024,40(7):226-234,9.基金项目
国家自然科学基金项目(31401799),山东省自然科学基金项目(ZR2020MC126),山东省研究生教育教学改革研究重点项目(SDYJG19056),山东省抗体制药协同创新中心开放课题(CICGAD1816),聊城大学畜牧学重点学科开放课题(319462207-23) （31401799）
