生物技术通报2024，Vol.40Issue(7)：247-258,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1183
不同覆盖处理对菠萝园土壤代谢物和细菌群落结构的影响
Analysis of Differential Metabolites and Bacterial Community Structure in the Soils of a Pineapple Orchard under Different Mulching Treatments
摘要
Abstract
[Objective]The aim of this work is to investigate the effects of different mulching treatments on the soil microecological environment in pineapple orchard.[Method]Taking the un-mulched soil as the control(CK),the differences in physicochemical properties,microbial abundance,bacterial community structure,and metabolites of the soils of mulching treatments with polyethylene mulch(PM),non-woven fabric(NW)and biodegradable film(BF)were compared and measured,respectively.[Result]Compared with CK,PM significantly increased the activity of protease,whereas,decreased the activities of catalase and sucrase.NW significantly increased the content of available P,and decreased the activities of catalase and sucrase.Similarly,BF significantly increased the contents of total N,available P,and the activity of acid phosphatase,and decreased soil pH and the activities of catalase and sucrase.Compared with CK,PM significantly increased the community of actinomycetes.NW significantly increased the community of bacteria,while decreased the actinomycetes.BF significantly increased the communities of bacteria and fungi,while decreased the actinomycetes.The bacterial 16S high-throughput sequencing profiling suggested that the diversity of CK was higher than those of the three mulching treatments,and the bacteria abundance of BF was the highest among the three treatments and CK.Metabolites analysis indicated that there were 17 differential accumulated metabolites in the soils of three comparison groups with CK,mainly including carbohydrates,acids,nitrogen compounds and alcohols.And BF accumulated the highest sugar metabolites in the three mulching treatments.CCA analysis showed that soil pH affected the differentiation of bacterial community structure,and soil nutrients and enzyme activities were positively correlated with bacterial community structure.[Conclusion]Among the three mulching treatments,NW would benefit to maintain the relatively stable microecological environment of soil during the vegetative growth period of pineapple.关键词
覆盖/菠萝/土壤理化性状/代谢物/细菌群落结构Key words
mulching-practices/pineapple/physiochemical properties of soil/soil metabolites/bacterial community structure引用本文复制引用
刘传和,贺涵,邵雪花,何秀古..不同覆盖处理对菠萝园土壤代谢物和细菌群落结构的影响[J].生物技术通报,2024,40(7):247-258,12.基金项目
国家重点研发计划(2023YFD2300800),广东省现代农业产业技术体系创新团队建设专项资金,广东省农村科技特派员项目(KTP20210395),广州市科技计划项目(2023E04J0562),广东省农业科学院菠萝产业研究院项目 （2023YFD2300800）
