生物技术通报2024，Vol.40Issue(7)：323-334,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0076
绵羊肺炎支原体GH3-3株全基因组测序及生物信息学分析
Whole Genome Sequencing and Bioinformatics Analysis of Mycoplasma ovipneumoniae GH3-3 Strain
摘要
Abstract
[Objective]This study is aimed to decode the genomic sequence of Mycoplasma ovipneumoniae strain GH3-3,to investigate its pathogenic potential and to elucidate the regulatory mechanisms of its replication,transcription,and translation.[Method]M.ovipneumoniae GH3-3 strain was cultured in vitro.When it reached the late logarithmic stage of growth,the genomic DNA of M.ovipneumoniae GH3-3 strain was extracted with bacterial genomic DNA extraction kit and sent for whole genome sequencing and commentary.[Result]The genome size of GH3-3 strains of M.ovipneumoniae was 1 060 772 bp,and the GC content was 29.66%.The genome component analysis showed that the genome of GH3-3 strain contained 730 coding genes,with a total length of 914 379 bp and an average length of 1 252.57 bp,accounting for 86.2%of the total genome length.There were 149 tandem repeats with a total length of 20 926 bp,accounting for 1.97%of the total length of the genome.There were 102 microsatellite DNA sequences,30 tRNA sequences,and 3 rRNA sequences.The 719,459,473,394,449,33,5,180,113 and 59 genes were annotated in NR,SwissProt,GOG,KEGG,GO,CARD,CAZy,PHI,TCDB,and RMS databases,respectively.A total of 76 virulence factor-related genes were annotated.The genome sequence was submitted to the NCBI website with the login number:PRJNA1051969.[Conclusion]The complete genome information of M.ovipneumoniae GH3-3 strain was acquired,and the gene function was predicted and annotated.Furthermore,the genetic and evolutionary relationships between GH3-3 strain and other strains of M.ovipneumoniae were elucidated globally.关键词
绵羊肺炎支原体/GH3-3株/全基因组测序/毒力因子/耐药基因Key words
Mycoplasma ovipneumoniae/GH3-3 strain/whole genome sequencing(WGS)/virulence factor/drug-resistant gene引用本文复制引用
田彤彤,葛家振,高鹏程,李学瑞,宋国栋,郑福英,储岳峰..绵羊肺炎支原体GH3-3株全基因组测序及生物信息学分析[J].生物技术通报,2024,40(7):323-334,12.基金项目
国家重点研发计划(2022YFD1800704),中国农业科学院兰州兽医研究所"元亨人才"计划项目(所级青年英才培育项目)(NKLS2020-119),甘肃省科技重大专项课题(23ZDNA007) （2022YFD1800704）
