山西大学学报（自然科学版）2024，Vol.47Issue(4)：874-884,11.DOI:10.13451/j.sxu.ns.2024061
虾青素对急性大强度运动大鼠心肌损伤影响的代谢组学研究
Metabolomic Study on the Effects of Astaxanthin on Myocardial Injury in Acute High-intensity Exercise Rats
摘要
Abstract
To investigate the effects of astaxanthin supplementation on myocardial metabolism in rats with acute high-intensity exer-cise by liquid chromatography-mass spectrometry(LC-MS).Twenty four seven-week-old healthy male SD(Sprague Dawley)rats were randomly divided into two exercise groups(group E)and an exercise administration group(group EM).The EM group was giv-en oil-soluble astaxanthin(25 mg/(kg·d))and the E group was given the same dose soybean oil.The exercise methods of both groups were:one-time acute high-intensity treadmill exercise;intervention for 4 weeks;All rats were sacrificed immediately after the last acute high-intensity exercise,and the blood and myocardial tissue was collected.The cTnI in blood,a marker of myocardial injury was detected,after sample processing,the corresponding metabolic map was detected by LC-MS technology.For example,XCMS,SIMCA-P,Excel,Origin,and other software were used for image and data processing and analysis.The differential metabo-lites were identified,and the target metabolic markers and metabolic pathways were screened by meT-PA and KEGG databases.The results showed that there were 15 kinds of differential metabolites in the myocardium of rats in the two groups,which were involved in 9 metabolic pathways.The glutathione metabolism,linoleic acid metabolism,cysteine,and methionine metabolism pathways were identified as three potential target pathways of astaxanthin.There were six markers involved in the target metabolic pathway:S-adenosyl methionine,serine 3-phosphate,SOPC,glutathione,and linoleic acid 5-L-glutamyl-L-alanine,they are related to the reduc-tion of oxidative stress and myocardial injury.The results showed that astaxanthin can regulate oxidative stress,improve myocardial injury,and protect the heart by regulating the metabolism of cysteine and methionine,linoleic acid,and glutathione.关键词
虾青素/运动/损伤/多组学Key words
astaxanthin/exercise/injury/multiomics分类
社会科学引用本文复制引用
郭新明,王佳,曹秀慧,高雅,王烨璠,吴丽君..虾青素对急性大强度运动大鼠心肌损伤影响的代谢组学研究[J].山西大学学报（自然科学版）,2024,47(4):874-884,11.基金项目
山西省重点研发计划项目(201803D31030) （201803D31030）
教育部人文社会科学研究项目(20YJA890025) （20YJA890025）
