虾青素对急性大强度运动大鼠心肌损伤影响的代谢组学研究OA北大核心CSTPCD

To investigate the effects of astaxanthin supplementation on myocardial metabolism in rats with acute high-intensity exer-cise by liquid chromatography-mass spectrometry(LC-MS).Twenty four seven-week-old healthy male SD(Sprague Dawley)rats were randomly divided into two exercise groups(group E)and an exercise administration group(group EM).The EM group was giv-en oil-soluble astaxanthin(25 mg/(kg·d))and the E group was given the same dose soybean oil.The exercise methods of both groups were:one-time acute high-intensity treadmill exercise;intervention for 4 weeks;All rats were sacrificed immediately after the last acute high-intensity exercise,and the blood and myocardial tissue was collected.The cTnI in blood,a marker of myocardial injury was detected,after sample processing,the corresponding metabolic map was detected by LC-MS technology.For example,XCMS,SIMCA-P,Excel,Origin,and other software were used for image and data processing and analysis.The differential metabo-lites were identified,and the target metabolic markers and metabolic pathways were screened by meT-PA and KEGG databases.The results showed that there were 15 kinds of differential metabolites in the myocardium of rats in the two groups,which were involved in 9 metabolic pathways.The glutathione metabolism,linoleic acid metabolism,cysteine,and methionine metabolism pathways were identified as three potential target pathways of astaxanthin.There were six markers involved in the target metabolic pathway:S-adenosyl methionine,serine 3-phosphate,SOPC,glutathione,and linoleic acid 5-L-glutamyl-L-alanine,they are related to the reduc-tion of oxidative stress and myocardial injury.The results showed that astaxanthin can regulate oxidative stress,improve myocardial injury,and protect the heart by regulating the metabolism of cysteine and methionine,linoleic acid,and glutathione.