甘草查尔酮A对HL-60细胞恶性生物学行为的抑制作用及其机制OACSTPCD

Objective To explore the effect of licochalcone A(LA)on acute myeloid leukemia(AML)HL-60 cell and its mechanism.Methods Human AML cell line HL-60 cells were divided into control group,LA group(30 μmol/L LA),IL-6 group(100 ng/mL IL-6,an activator of JAK2/STAT3 signaling pathway)and LA+IL-6 group.After 48 h culture,the proliferation activity of HL-60 cells was detected by MTT assay and clonal formation assay.The cell cycle distribution and the apoptosis of HL-60 cells were detected by flow cytometry.The invasion and migration abilities of HL-60 cells were detected by Transwell assay.The protein expression levels of p21,p27,cyclin E2,CDK2,Bcl-2,Bax,cleaved Caspase-3,p-JAK2,JAK2,p-STAT3 and STAT3 were detected by Western blot.Results Compared with control group,the cell viability,the number of clonal formation,the numbers of invasion and migration cells were decreased in LA group(P＜0.05),the protein expression levels of Bcl-2,cyclin E2 and CDK2 were decreased(P＜0.05),the phosphorylation levels of JAK2 and STAT3 were decreased(P＜0.05),the proportion of cells in G0/G1 phase and the apoptosis rate were increased(P＜0.05),and the protein expression levels of p21,p27,Bax and cleaved Caspase-3 were increased(P＜0.05).Compared with control group,the cell viability,the number of clonal formation,the numbers of invasion and migration cells were increased in IL-6 group(P＜0.05),the protein expression levels of Bcl-2,cyclin E2 and CDK2 were increased(P＜0.05),the phos-phorylation levels of JAK2 and STAT3 were increased(P＜0.05),the proportion of cells in G0/G1 phase and the apoptosis rate were decreased(P＜0.05),and the protein expression levels of p21,p27,Bax and cleaved Caspase-3 were decreased(P＜0.05).Com-pared with IL-6 group,the cell viability,the number of clonal formation,the numbers of invasion and migration cells were decreased in LA+IL-6 group(P＜0.05),the protein expression levels of Bcl-2,cyclin E2 and CDK2 were decreased(P＜0.05),the phosphoryla-tion levels of JAK2 and STAT3 were decreased(P＜0.05),the proportion of cells in G0/G1 phase and the apoptosis rate were inereased(P＜0.05),and the protein expression levels of p21,p27,Bax and cleaved Caspase-3 were increased(P＜0.05).There was no significant difference in the above indexes between LA+IL-6 group and LA group.Conclusion LA can inhibit the prolifera-tion,invasion and migration abilities of AML cell line HL-60 cells,induce the cell apoptosis and the cell arrest in GO/G1 phase,which may be caused by inhibiting the activation of JAK2/STAT3 signaling pathway.