山西医科大学学报2024，Vol.55Issue(6)：731-734,4.DOI:10.13753/j.issn.1007-6611.2024.06.008
阴道乳杆菌抑制加德纳菌的机制研究
Study on the mechanism of vaginal Lactobacillus inhibiting Gardnerella
摘要
Abstract
Objective To investigate the inhibitory effect of Lactobacillus isolated from vaginal microenvironment of healthy Chinese women on Gardnerella vaginalis isolated from vaginal microenvironment of patients with bacterial vaginosis and its mechanism.Methods Vaginal Lactobacillus strains from healthy Chinese women and Gardnerella vaginalis strains from patients with bacterial vaginosis were co-cultured in vitro to evaluate the effect of Lactobacillus on the proliferation activity of Gardnerella vaginalis.Vaginal Lactobacillus and Gardnerella vaginalis were co-cultured together with vaginal epithelial cells,and the adhesion of Gardnerella vaginalis to epithelial cells was calculated to evaluate the effect of Lactobacillus on the adhesion of Gardnerella vaginalis to epithelial cells.The inhibitory effects of Lactobacillus culture solutions,bacteria,supernatant,vaginal epithelial cells,Lactobacillus+vaginal epithelial cells and negative control(MRS broth)on Gardnerella were compared to analyze the possible mechanism of Lactobacillus inhibiting Gardnerella.Results A total of 5 strains of Lactobacillus(Lactobacillus crispatus,Lactobacillus gasseri,Lactobacillus jensenni,Lactobacillus fermen-tum,Lactobacillus delbrueckki)had a significant inhibitory effect on the proliferative activity of Gardnerella vaginalis(P＜0.05),and Lactobacillus crispatus,Lactobacillus gasseri,and Lactobacillus jensenni significantly interfered with the adhesion process of Gardnerella to epithelial cells(P＜0.05).The antibacterial effect of bacterial cells,epithelial cells,supernatant,and bacterial liquid gradually increased,and the co-culture of bacterial cells and epithelial cells had higher antibacterial effect than that of bacterial solution.Conclusion Vaginal Lactobacillus strains from healthy women can inhibit the activity of Gardnerella vaginalis in patients with bacterial vaginosis,and interfere with the adhesion process of Gardnerella vaginalis to vaginal epithelial cells.The inhibitory effect of Lactobacillus on Gardnerella is accomplished via multiple mechanisms.关键词
阴道乳杆菌/阴道加德纳菌/细菌性阴道病/抑菌功能/细菌黏附性Key words
vaginal Lactobacillus/Gardnerella vaginalis/bacterial vaginosis/bacteriostatic function/bacterial adhesion分类
医药卫生引用本文复制引用
张瑞,张晓宇,马爱昕,刘朝晖..阴道乳杆菌抑制加德纳菌的机制研究[J].山西医科大学学报,2024,55(6):731-734,4.基金项目
国家自然科学基金项目(81471420) （81471420）
教育部高等学校博士学科点专项科研基金项目(20130001120051) （20130001120051）
