基于咽部分泌物蛋白质组学分析的术后咽痛机制研究OACSTPCD

Objective To screen the differentially expressed proteins in postoperative sore throat(POST),and investigate the pathoge-nesis of POST.Methods The pharyngeal secretions from 20 patients who underwent elective surgery under general anesthesia with endotracheal intubation in the First Hospital of Shanxi Medical University were collected.Based on whether the patients suffered pha-ryngeal pain within 24 h after surgery,the patients were divided into POST group and non-POST group(control group),with 10 cases in each group.The protein quantitative analysis was conducted to screen the differential protein spectra in the samples,and the diffe-rential proteins were analyzed by gene ontology(GO)annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.Results A total of 3 419 proteins were identified in both POST group and control group,of which 190 proteins were signifi-cantly upregulated and 16 proteins were significantly downregulated.GO annotation demonstrated that the differential proteins were mainly involved in cellular metabolism,biological regulation,stress response,and immune processes.KEGG enrichment analysis showed that the differential proteins were mainly enriched in Wnt,ubiquitin-mediated proteolysis,and oxidative phosphorylation signaling pathways.Conclusion Postoperative sore throat may involve in many biological processes,among which the Wnt signaling pathway is closely correlated with the pain,suggesting that POST can be medicated by Wnt signaling pathway.