山西医科大学学报2024，Vol.55Issue(6)：753-762,10.DOI:10.13753/j.issn.1007-6611.2024.06.012
黄芪注射液中初生成分的鉴定及氨基酸类成分的含量测定
Identification of primary components and assay of amino acids in Astragalus injections
摘要
Abstract
Objective To identify the primary components in Astragalus injection and evaluate the quality differences of Astragalus injections from different manufacturers based on the contents of amino acids.Methods The primary components in Astragalus injec-tion were detected by gradient elution using 0.1％ acetonitrile formate and 0.1％ formic acid water as mobile phase by LC-MS/MS method.With 10％methanol water(containing 0.1％ formic acid)and 50％ methanol water(containing 0.1％ formic acid)as mobile phases,the content of each amino acid was determined after gradient elution,and the quality difference of amino acids in the primary components of Astragalus injections from different manufacturers was evaluated by principal component analysis.Results A total of 255 primary components were detected in all Astragalus injections from different manufacturers,including amino acids,organic acids,sugars and N-containing compounds.And the score of amino acid content in four manufacturers was-0.23,-1.77,-0.35,2.36,respectively.Conclusion The types of primary components in Astragalus injection from different manufacturers are the same,but the contents of primary components are different.The contents of amino acids in Astragalus injections from different manufacturers vary greatly.关键词
黄芪注射液/初生成分/成分鉴定/含量测定/液相二级质谱Key words
Astragalus injection/primary components/ingredient identification/content determination/LC-MS/MS分类
医药卫生引用本文复制引用
曲婷丽,闫丽秋,周小雪,王文涛,张楠,蔡均杰..黄芪注射液中初生成分的鉴定及氨基酸类成分的含量测定[J].山西医科大学学报,2024,55(6):753-762,10.基金项目
山西省科技厅基础研究项目(20210302124586) （20210302124586）
太原市科技局"双百攻关行动"首批关键核心技术攻关"揭榜挂帅"项目(2024TYJB0150) （2024TYJB0150）
山西省重点研发计划项目(202102130501023) （202102130501023）
