陕西医学杂志2024，Vol.53Issue(7)：867-872,6.DOI:10.3969/j.issn.1000-7377.2024.07.001
细颗粒物毒性组分对血管平滑肌细胞增殖的影响及其机制研究
Effect and mechanism of toxic components of fine particulate matter on proliferation of vascular smooth muscle cells
摘要
Abstract
Objective:To investigate the effect of typical toxicity of fine particulate matter(PM2.5)on the proliferation of vascular smooth muscle cells and its mechanism.Methods:The whole particles,liposoluble-soluble and water-soluble PM2.5 were prepared respectively.Vascular smooth muscle cells(VSMCs)were co-cultured with different components of PM2.5 and mitogen-activated protein kinase(MAPK)signaling pathway inhibitors.The effects of PM2.5 on the proliferation of VSMCs were evaluated by MTT method.The effects of different components of PM2.5 on inflammatory factors were de-termined by ELISA.The expression of MAPK proteins in VSMCs were detected by immunefluore scence assay.Results:The whole particles PM2.5(WPPM2.5)and liposoluble-soluble,PM2.5(LSPM2.5)inhibited the proliferation of VSMCs,and the IC50 values of 24 hours were 178.3 and 283.9 μg/ml,respectively.LSPM2.5 could significantly enhance the expression of IL-8.U0126,SB2035850 and SP600125(MAPK signaling pathway inhibitors)could significantly inhibit the increase on IL-8,antago-nize the inhibition of WPPM2.5 and LSPM2.5 on VSMCs,and increase the cell survival rate.WPPM2.5 and LSPM2.5 activated the phosphorylation of P38,JNK and ERK 1/2 signaling pathways in VSMCs,and increased the expression of phosphorylated pro-teins.Conclusion:PM2.5 inhibit VSMCs through MAPK inflammation signaling pathway,which is mainly related to its whole and liposoluble-soluble components.关键词
细颗粒物/组分/血管平滑肌细胞/细胞毒性/炎性反应/丝裂原活化蛋白激酶信号通路Key words
PM2.5/Components/Vascular smooth muscle cells/Cytotoxicity/Inflammatory response/MAPK signaling pathway分类
医药卫生引用本文复制引用
王荣,王娜娜,张懿烨,陈倩,杨宽,秦蓓..细颗粒物毒性组分对血管平滑肌细胞增殖的影响及其机制研究[J].陕西医学杂志,2024,53(7):867-872,6.基金项目
国家自然科学基金资助项目(81903288) （81903288）
陕西省重点研发计划项目(2021ZDLSF03-05) （2021ZDLSF03-05）
西安医学院校级科技创新团队项目(2021TD03) （2021TD03）
教育部产学合作协同育人项目(202101021043,220605634241456) （202101021043,220605634241456）
西安医学院大学生创新创业训练计划项目(121523020) （121523020）
