陕西医学杂志2024，Vol.53Issue(7)：884-889,6.DOI:10.3969/j.issn.1000-7377.2024.07.004
长链非编码RNASUCLG2-AS1通过调节微小RNA-491-5p对非小细胞肺癌细胞增殖和免疫逃逸的影响
Effects of lncRNA SUCLG2-AS1 on non-small cell lung cancer cell proliferation and immune evasion by regulating miR-491-5p
摘要
Abstract
Objective:To explore the effect of long non-coding RNA(lncRNA)SUCLG2-AS1 on the prolifera-tion and immune evasion of non-small cell lung cancer cells and its regulatory effect on miR-491-5p.Methods:The Cancer LncRNA Census database was used to analyze the expression of SUCLG2-AS1 in non-small cell lung cancer tissues and the relationship between SUCLG2-AS1 expression and the survival of patients with non-small cell lung cancer.The expression changes of SUCLG2-AS1 in non-small cell lung cancer lines A549,PG49,H1975,H2170 and human normal lung bronchial epithelial cells BEAS-2B were detected by qRT-PCR.H1975 cells were cultured and di-vided into si-NC group and si-SUCLG2-AS1 group.Colony experiment and flow cytometry were used to detect the proliferation ability and cell cycle of H1975 cells.Western blot was used to detect the expression of immune-related proteins programmed cell death 1 ligand 1(PD-L1),RAR-related orphan receptor(RORγt)and cell cycle-related pro-teins cyclin dependent kinase3(Cdk3),Cyclin C,and cyclin dependent kinase2(Cdk2)in H1975 cells.LncRMap bioinformatics software was used to predict microRNA that SUCLG2-AS1 can bind.RNA pull-down experiments was used to verify the direct binding of SUCLG2-AS1 to miR-491-5p.The regulatory effect of SUCLG2-AS1 on the expression of miR-491-5p was detected by qRT-PCR.Results:Compared with normal lung tissue,the expression of SUCLG2-AS1 was increased in non-small cell lung cancer tissue(P＜0.01).The survival time of non-small cell lung cancer patients with high SUCLG2-AS1 expression was shorter than that of patients with low SUCLG2-AS1 expres-sion(P＜0.01).Compared with BEAS-2B cells,the expression of SUCLG2-AS1 was increased in non-small cell lung cancer lines,and the expression of SUCLG2-AS1 was most significantly increased in H1975 cells(all P＜0.05).Compared with the si-NC group,the proliferation ability of H1975 cells in the si-SUCLG2-AS1 group decreased,the proportion of cells in G0/G1 phase increased,the proportion of cells in S phase decreased,and the proportion of cells in G2/M phase decreased(all P＜0.01).Compared with the si-NC group,the expression of immune-related proteins PD-L1 and RORyt in H1975 cells in the si-SUCLG2-AS1 group was reduced,and the expression of cell cycle-related proteins Cdk3,Cyclin C,and Cdk2 was reduced.SUCLG2-AS1 targets and directly binds to miR-491-5p(all P＜0.01).Compared with the si-NC group,the expression of miR-491-5p in H1975 cells in the si-SUCLG2-AS1 group increased(P＜0.01).Conclusion:Silencing SUCLG2-AS1 may inhibit the proliferation and immune evasion of non-small cell lung cancer cells by upregulating the expression of miR-491-5p.关键词
非小细胞肺癌/SUCLG2-AS1/微小RNA-491-5p/细胞增殖/细胞周期/免疫逃逸Key words
Non-small cell lung cancer/SUCLG2-AS1/miR-491-5p/Cell proliferation/Cell cycle/Immune escape分类
医药卫生引用本文复制引用
陈娟,郭金莲,周慧会,刘琳,杨柳,张华..长链非编码RNASUCLG2-AS1通过调节微小RNA-491-5p对非小细胞肺癌细胞增殖和免疫逃逸的影响[J].陕西医学杂志,2024,53(7):884-889,6.基金项目
国家自然科学基金资助项目(81860413) （81860413）
