陕西医学杂志2024，Vol.53Issue(7)：895-899,5.DOI:10.3969/j.issn.1000-7377.2024.07.006
长链非编码RNA母系表达基因8通过微小RNA-495-3p调控急性髓性白血病细胞高迁移率族蛋白A1表达及对细胞增殖、凋亡的作用机制研究
LncRNA MEG8 regulates HMGA1 expression and the mechanism of cell proliferation and apoptosis in acute myeloid leukemia cells through miR-495-3p
摘要
Abstract
Objective:To investigate the mechanism of long non-coding RNA(lncRNA)maternal expression gene 8(MEG8)regulating the expression of high mobility group protein 1(HMGA1)through miR-495-3p in acute myeloid leukemia(AML)cells,cell proliferation and apoptosis.Methods:Bone marrow biopsy samples from 50 pa-tients diagnosed with AML and bone marrow samples from 52 healthy bone marrow donors were selected.Human AML cell line HL-60 was cultured routinely,and lncRNA MEG8 mRNA was expressed in bone marrow mononuclear cells by RT-qPCR method.The HL-60 cells were divided into six groups.That is,HL-60 group(HL-60 cells),si-NC group(transfected si-NC),si-lncRNA MEG8 group(transfected si-lncRNA MEG8),mimeo-NC group(transfected Mimeo-NC),miR-495-3p mimic group(transfected miR-495-3p mimic group)and si-lncRNA MEG8+miR-495-3p mimic group(transfected with si-lncRNA MEG8+miR-495-3p mimic group),the cell proliferation ability of each group was detected by CCK-8 method after 24,48,and 72 hours.Cell apoptosis was detected by flow cytometry.Western blot was used to detect HMGA1 expression in cells.Dual luciferase reporter gene assay verified the relation-ship between lncRNA MEG8,miR-495-3p and HMGA1.Results:Compared with control group,lncRNA MEG8 mRNA expression in observation group was increased(P＜0.05).The luciferase activity of miR-495-3p mimic+MEG8 WT co-transfection group decreased compared with that of miR-NC+MEG8 WT co-transfection group(P＜0.05).Compared with that of miR-NC+HMGA1 WT co-transfection group,the luciferase activity of miR-495-3p mimic+MEG8 WT co-transfection group decreased significantly(P＜0.05).The luciferase activity of miR-495-3p mimic+HMGA1 WT co-transfected group decreased(P＜0.05).Cell proliferation ability of si-lncRNA MEG8 group,miR-495-3p mimic group and si-lncRNA MEG8+miR-495-3p mimic group decreased significantly at 24 and 48 hours compared with HL-60 group,si-NC group and mimic-NC group(all P＜0.05).The cell proliferation rate of si-ln-cRNA MEG8+miR-495-3p mimic group was the lowest(P＜0.05).The apoptosis rate of HL-60 cells in si-lncRNA MEG8+miR-495-3p mimic group was higher than that in si-lncRNA MEG8 group and miR-495-3p mimic group compared with HL-60 group,si-NC group and MImea-NC group(all P＜0.05).Compared with HL-60 group,si-NC group and mimic-NC group,the HMGA1 protein expression of si-lncRNA MEG8+miR-495-3p mimic group was lower than that of si-lncRNA MEG8 group and miR-495-3p mimic group(all P＜0.05).Conclusion:Silencing lncRNA MEG8 may down-regulate HMGB1 through up-regulation of miR-495-3p,thereby inhibiting the malignant biological behavior of HL-60 cells,providing a new idea for exploring potential therapeutic targets for AML.关键词
长链非编码RNA/母系表达基因8/微小RNA-495-3p/急性髓性白血病细胞/高迁移率族蛋白1Key words
Long non-coding RNA/Maternal expression gene 8/miR-495-3p/Acute myeloid leukemia cells/High mobility group protein 1分类
医药卫生引用本文复制引用
张璐,郭含梦,王庆义..长链非编码RNA母系表达基因8通过微小RNA-495-3p调控急性髓性白血病细胞高迁移率族蛋白A1表达及对细胞增殖、凋亡的作用机制研究[J].陕西医学杂志,2024,53(7):895-899,5.基金项目
安徽省自然科学基金资助项目(2208085MH217) （2208085MH217）
