陕西医学杂志2024，Vol.53Issue(7)：900-904,909,6.DOI:10.3969/j.issn.1000-7377.2024.07.007
炎性细胞因子在射频导管消融术治疗房颤大鼠中的表达及对心肌损伤的影响
Expression of inflammatory cytokines in rats with atrial fibrillation treated by radiofrequency catheter ablation and its effect on myocardial injury
摘要
Abstract
Objective:To investigate the expression of inflammatory cytokines in rats with atrial fibrillation trea-ted by radiofrequency catheter ablation and its effect on myocardial injury.Methods:Sixty 6-week-old SPF male SD rats with a body weight of 180 to 200 g were purchased and randomly divided into blank group,model group and RF catheter ablation group with 20 rats each.The blank group was fed normally for 1 week.Atrial fibrillation animal models were established in both model group and RF catheter ablation group.After feeding the blank group for 1 week,50 μg/100 g0.9％sodium chloride was injected through the rat tail.After successful modeling in the model group,calcium chloride(10 mg/ml)was mixed with acetylcholine(66 μg/ml)and injected through rat tail vein.RF catheter ablation group had the same method of atrial fibrillation modeling as the model group,and 3 days after suc-cessful modeling,radiofrequency catheter ablation was given.According to the level of cardiac troponin T(CTnT)af-ter operation,the rats were divided into myocardial injury group(7 rats,more than 0.08 ng/L)and non-myocardial injury group(12 rats,less than 0.08 ng/L).Results:After modeling in the model group and the RF catheter ablation group,2 of 40 rats died,resulting in a success rate of 95.00％(38/40).Finally,19 rats in the model group and 19 rats in the RF catheter ablation group were successfully modeled.The rats in the blank group had glossy fur,normal diet and water,even breathing and sensitive activity.The rats in the model group had dull hair color,bloodshot eyes,reduced diet and water,accelerated respiration and heart rate,slow movement,fatigue and dull eyes.The rats in the radio frequency catheter ablation group had no obvious loss of appetite,slightly accelerated heart rate and breathing,bloodshot eyes,and sleepy.The duration and induction time of atrial fibrillation in the model group were higher than those in the non-myocardial injury group and the myocardial injury group,and the duration and induction time of atri-al fibrillation in the myocardium injury group were higher than those in the non-myocardial injury group(all P＜0.05).In the blank group,the myocardial cells were normal,neatly arranged and dense,and the cell morphology was normal.In the model group,the interstitium of myocardial cells increased,the space increased,the arrangement was disordered,and there was a lot of inflammatory infiltration.In the non-myocardial injury group,the interstitial tissue of myocardium increased slightly,the arrangement was orderly,and there was no obvious inflammatory cell infiltra-tion.In the myocardial injury group,the interstitium of myocardium increased significantly,the space increased slightly,the arrangement was slightly disordered,and there was inflammatory infiltration.The levels of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in myocardial injury group were higher than those in blank group and non-myocardial injury group,and the levels of IL-1β,IL-6 and TNF-α in model group and non-my-ocardial injury group were higher than those in blank group.The levels of IL-1β,IL-6 and TNF-α in the model group were higher than those in the non-myocardial injury group(all P＜0.05).Serum creatine kinase isoenzyme(CK-MB),nitric oxide(NO),nitric oxide synthase(iNOS)and cysteine aspartic protease-3(Caspase-3)levels in myocardial injury group were higher than those in blank group and non-myocardial injury group.The levels of CK-MB,NO,iNOS and Caspase-3 in model group and non-myocardial injury group were higher than those in blank group,and the levels of CK-MB,NO,iNOS and Caspase-3 in model group were higher than those in non-myocardial in-jury group(all P＜0.05).Conclusion:IL-1β,IL-6 and TNF-α are highly expressed in rats with atrial fibrillation myocardial injury,and the mechanism is that these inflammatory cytokines can regulate the function and apoptosis of cardiomyocytes.关键词
白细胞介素-1β/白细胞介素-6/肿瘤坏死因子-α/血清肌酸激酶同工酶/一氧化氮/一氧化氮合酶/半胱氨酸天冬氨酸蛋白酶-3/房颤/心肌损伤Key words
IL-1β/IL-6/TNF-α/CK-MB/NO/iNOS/Caspase-3/Atrial fibrillation/Myocardial injury分类
医药卫生引用本文复制引用
张冬青,潘飞,胡梦婷,干佳琦,邵金炎..炎性细胞因子在射频导管消融术治疗房颤大鼠中的表达及对心肌损伤的影响[J].陕西医学杂志,2024,53(7):900-904,909,6.基金项目
上海市闵行区卫生健康委员会临床研究类科研立项课题(2021MW67) （2021MW67）
上海市中西医结合学会科研基金资助项目(2021-13) （2021-13）
上海市闵行区中心医院扶持学科-医联体慢病管理项目(YJXK-2021-15) （YJXK-2021-15）
