陕西医学杂志2024，Vol.53Issue(7)：905-909,5.DOI:10.3969/j.issn.1000-7377.2024.07.008
依托咪酯对视神经损伤成年大鼠视网膜神经节细胞的保护作用及对半胱氨酸天冬氨酸蛋白酶3、脑源性神经营养因子蛋白表达的影响
Protective effect of etomidate on retinal ganglion cells and its effect on expression of Caspase-3 and brain-derived neurotrophic factor in adult rats with optic nerve injury
摘要
Abstract
Objective:To investigate the protective effect of etomidate on retinal ganglion cells and its effect on expression of Caspase-3 and brain-derived neurotrophic factor(BDNF)in adult rats with optic nerve injury.Methods:A total of 40 adult male SD rats were selected,8 were randomly selected as normal group,and the other 32 rats were treated with optic nerve injury by artery clamp method to establish optic nerve injury model and divided into model group(optic nerve injury rats)and low,medium and high dose etomidate group(intraperitoneal injection of etomid-ate)with dose of 2,4 and 6 mg/kg,respectively.The changes of intraocular pressure in each group were compared after intervention.The retinal tissue structure was observed by HE staining,and the number and survival rate of reti-nal ganglion cells(RCGs)in each group were compared,and the protein expressions of Caspase-3 and BDNF in reti-nal tissues were detected by Western blot.Results:The IOP in the model group and etomidate group was higher than that in the normal group,and the IOP in the etomidate group decreased after the last administration in a dose-depend-ent manner(all P＜0.05).In the model group,retinal edema and thickening were most obvious in the nerve fiber lay-er,with vacuoles,swelling of RGC cells,a small decrease in the inner and outer nuclear layer cells,and disordered ar-rangement.The retinal pathological injury was improved in all etomidate groups,and the high dose group was better than the medium dose group,and the medium dose group was better than the low dose group.Compared with the normal group,the surviving number of RCGs in the model group was decreased(P＜0.05).Compared with the mod-el group,the surviving number of RCGs in the etomidate groups was increased(P＜0.05).The number and relative survival rate of RCGs in high dose etomidate group were higher than those in medium and low dose groups(all P＜0.05).The expression of BDNF protein in retinal tissues of normal group was higher than that of model group,Caspase-3 was lower than that of model group,BDNF was increased and Caspase-3 was decreased in retinal tissues of etomidate group,all in a dose-dependent manner(all P＜0.05).Conclusion:The expression of Caspase-3 protein is increased and the expression of BDNF protein is decreased in rats with optic nerve injury.Etomidate intervention can promote the survival of retinal RGCs and has a protective effect on optic nerve injury.关键词
依托咪酯/视神经损伤/视网膜神经节细胞/半胱氨酸天冬氨酸酶3/脑源性神经营养因子/大鼠Key words
Etomidate/Optic nerve injury/Retinal ganglion cells/Cysteinyl aspartate specific protease 3/Brain-derived neurotrophic factor/Rat分类
医药卫生引用本文复制引用
赵翠党,赵红雷,史炯,赵广平..依托咪酯对视神经损伤成年大鼠视网膜神经节细胞的保护作用及对半胱氨酸天冬氨酸蛋白酶3、脑源性神经营养因子蛋白表达的影响[J].陕西医学杂志,2024,53(7):905-909,5.基金项目
河北省卫生健康委员会科研基金资助项目(20191850) （20191850）
