陕西医学杂志2024，Vol.53Issue(7)：970-973,977,5.DOI:10.3969/j.issn.1000-7377.2024.07.023
房颤患者射频消融术后血浆微小RNA-21、微小RNA-150水平变化及其检测价值分析
Changes of plasma miR-21 and miR-150 levels in patients with atrial fibrillation after radiofrequency ablation and their detection value analysis
摘要
Abstract
Objective:To explore the changes and detection value of plasma microRNA-21(miR-21)and mi-croRNA-150(miR-150)levels in patients with atrial fibrillation(AF)after radiofrequency ablation(RFA).Methods:86 patients with AF were selected as the observation subjects.According to whether AF recurred after RFA,they were divided into recurrence group(n=22)and non-recurrence group(n=64).The levels of plasma miR-21 and miR-150 were measured by real-time fluorescence quantitative polymerase chain reaction(PCR).The differ-ences in expressions of plasma miR-21 and miR-150 were compared among the subjects before and after surgery and between recurrence group and non-recurrence group.Spearman correlation was used to analyze the correlation be-tween plasma miR-21 and miR-150 levels after RFA and AF recurrence.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of plasma miR-21 and miR-150 levels on AF recurrence after RFA.Results:The level of plasma miR-21 after RFA was lower than that before surgery,while the level of miR-150 was higher than that before surgery(all P＜0.05).The plasma miR-21 level in recurrence group after RFA was higher,while the miR-150 level was lower compared to non-recurrence group(all P＜0.05).Spearman correlation analysis found that plasma miR-21 level after RFA was positively correlated with recurrence,and miR-150 level was negatively correlated with recurrence(all P＜0.05).ROC curve analysis showed that plasma miR-21,miR-150 levels and combined detection after RFA had predictive efficiency on AF recurrence(all P＜0.05),and the areas under curves(AUC)were 0.783,0.675 and 0.812 respectively.The combined test had the highest predictive efficiency,with sensitivity of 86.36％and specificity of 67.19％.Conclusion:The level of plasma miR-21 is decreased and the level of miR-150 is increased in AF patients after RFA,and the levels of miR-21 and miR-150 after surgery are correlated with AF recurrence.Combined detection of plasma miR-21 and miR-150 levels after RFA can predict the recurrence of AF after cardioversion.关键词
射频消融术/心房颤动/微小RNA-21/微小RNA-150/复发/预测价值Key words
Radiofrequency ablation/Atrial fibrillation/miR-21/miR-150/Recurrence/Predictive value分类
医药卫生引用本文复制引用
周晚晴,戴敏,孙娟娟,李靓..房颤患者射频消融术后血浆微小RNA-21、微小RNA-150水平变化及其检测价值分析[J].陕西医学杂志,2024,53(7):970-973,977,5.基金项目
安徽省重点研究与开发计划项目(202104j07020059) （202104j07020059）
