肺浸润性腺癌中Prominin2表达特征及对细胞增殖和凋亡调控的作用研究OACSTPCD

Objective:To detect the expression of Prominin2(PROM2)in lung invasive adenocarcinoma,analyze its clinical significance,and explore its regulation of cell proliferation and apoptosis.Methods:Bioinformatic analysis was applied to predict the difference of PROM2 expression in lung invasive adenocarcinoma and normal lung tissues.Fifty-eight patients diagnosed with lung invasive adenocarcinoma were selected,and postoperative tumor tissues and surrounding normal lung tissues were collected.Expressions of PROM2,proliferating cell nuclear antigen(PCNA),Bcl-2 associated X protein(BAX),B-cell lymphoma/leukemia-2(Bcl-2)and Caspase-3 were detected by immunohis-tochemistry.Human lung adenocarcinoma cell line A549 and human immortalized lung epithelial cell line 16HBE were selected.Human lung adenocarcinoma cell line A549 and human immortalized lung epithelial cell line 16HBE were selected.Small interfering RNA-PROM2 was synthesized and transfected into A549 cell line to establish si-RNA-PROM2(si-PROM2)group,and empty vector transfection group(si-NC)and blank control group(NC)were set up.The expressions of PROM2,PCNA,BAX,Bcl-2 and Caspase-3 were examined by Western blot,and the cell proliferation activity was detected by CCK-8 assay,and the apoptosis rate was detected by flow cytometry.Results:GEPIA database predicts that the expression of PROM2 in lung adenocarcinoma may be higher than that in normal lung tissue.The positive rate of PROM2 was significantly higher in lung invasive adenocarcinoma than that in normal lung tissues(P＜0.05).The expression of PROM2 was statistically different in different maximum tumor di-ameter and with or without dirty pleural involvement in lung invasive adenocarcinoma(all P＜0.05).The expression of PROM2 was not statistically different in sexes,ages,lymph node metastases and pathological types(all P＞0.05).There was a positive correlation between PROM2 and PCNA,PROM2 and Bcl-2,and a negative correlation between PROM2 and BAX,PROM2 and Caspase-3 in lung invasive adenocarcinoma(all P＜0.05).The expression of PROM2 was significantly higher in lung adenocarcinoma cell line A549 than that in human immortalized lung epithe-lial cell line(P＜0.05).The expressions of PCNA and Bcl-2 were significantly lower in the si-PROM2 group than those in the si-NC and NC groups,and the expressions of BAX and Caspase-3 were significantly higher than those in the si-NC and NC groups on A549(all P＜0.05).The proliferative activity was significantly lower in the si-PROM2 group than that in the si-NC and NC groups at 48 and 72 hours,and the apoptosis rate was significantly higher in the si-PROM2 group than that in the si-NC and NC groups(all P＜0.05).Conclusion:The expression of PROM2 was el-evated in lung invasive adenocarcinoma,which is involved in the formation and progression of lesions.PROM2 may play a biological role by promoting cell proliferation and inhibiting apoptosis.