实用妇产科杂志2024，Vol.40Issue(7)：554-559,6.
PD-1/PD-L1调控蜕膜巨噬细胞在复发性流产中的相关研究
Studies on PD-1/PD-L1 Regulation of Decidual Macrophages in Recurrent Spontaneous Abortion
摘要
Abstract
Objective:To investigate the impact of programmed death receptor-1(PD-1)and its ligand(PD-L1)on the differentiation of decidual macrophages(DMs)and embryonic development.Methods:20 patients with re-current spontaneous abortion(RSA group)and 20 patients with normal pregnancy induced artificial abortion(NP group)were selected from Sichuan Provincial People's Hospital between March 2021 and January 2024.Villous and decidual tissues were obtained from both groups.Real-time fluorescent quantitative polymerase chain reac-tion(qPCR)and Western blot techniques were used to detect the expression of PD-L1 mRNA and protein in vil-lous tissues.Flow cytometry was used to analyze the types of DMs and their PD-1 expression.Human monocytic leukemia cells(THP-1)were differentiated under different conditions,with a control group and a PD-1 blocked group established,and different reagents were added into those two groups to induce differentiation,then the pro-portion of DMs types was measured in both groups.Results:PD-L1 protein and mRNA were expressed in the vil-lous tissue of both groups of patients but the expression of PD-L1 protein and mRNA was reduced in the RSA group compared to the NP group(P<0.05).The decidual tissues of both groups contained M1 and M2 macro-phages.Compared with the NP group,the ratio of M2 to M1 DMs in the RSA group was lower(P<0.05),indica-ting a polarization towards the M1 type in DMs.There was no statistically significant difference in PD-1 expression between the two groups(P>0.05).Human THP-1 cells can be stimulated to differentiate into M1 and M2 macro-phages.Afterblocking PD-1,theratioof M2 to M1 macrophages decreased(P<0.05),indicating a polarization towards the M1 type in macrophages.Conclusions:In patients with RSA,the expression of PD-L1 in villous tis-sues is decreased,and DMs polarize towards the M1 type.Blocking the PD-1/PD-L1 pathway can further promote the polarization of macrophages towards the M1 type.The influence of PD-1/PD-L1 pathway on macrophage po-larization may provide new insights into the pathogenesis of RSA.关键词
程序性死亡受体-1/程序性死亡受体-1配体/蜕膜巨噬细胞/复发性流产Key words
Programmed death receptor-1/Programmed death receptor-1 ligand/Decidual macrophages/Re-current spontaneous abortion分类
医药卫生引用本文复制引用
毛伴云,张夏兰,税国鑫,梅劼,岳军..PD-1/PD-L1调控蜕膜巨噬细胞在复发性流产中的相关研究[J].实用妇产科杂志,2024,40(7):554-559,6.基金项目
四川省科技厅重点研发项目(编号:2021YFS0209) （编号:2021YFS0209）
