沈阳医学院学报2024，Vol.26Issue(4)：360-369,375,11.DOI:10.16753/j.cnki.1008-2344.2024.04.005
基于网络药理学与分子对接技术探讨保肾灵丸治疗糖尿病肾病的机制
Network pharmacology and molecular docking technology reveal the mechanism of kidney-protecting spirit pill in the treatment of diabetic nephropathy
摘要
Abstract
Objective:To explore the mechanism of kidney-protecting spirit pill for the treatment of diabetic nephropathy(DN)based on the network pharmacology and molecular docking technology.Methods:The database of TCMSP and Swiss Target Prediction was searched to obtain the active ingredients and targets of kidney-protecting spirit pill,and the intersection with the disease targets was obtained.The protein-protein interaction(PPI)network of intersection targets was constructed,GO and KEGG enrichment were analyzed.The key targets and small molecules were obtained and their interactions were verified by molecular docking.Results:A total of 60 active ingredients and 112 therapeutic DN targets were predicted.The key components were Cerevisterol,3,9-di-O-methylnissolin,Jaranol,Palmidin A and 16α-Hydroxydehydrotrametenolic acid.The key targets were PIK3CA,MAPK1,AKT1,PIK3R1 and BCL2,which were closely related to cancer-related pathways,AGE-RAGE signaling pathway,endocrine resistance,lipids and atherosclerosis pathways in diabetic complications.Conclusion:The mechanism of kidney-protecting spirit pill in the treatment of DN is characterized by multi-components,multi-targets and multi-pathways,with synergistic effects between the herbs,which provides a basis for the study of the pharmacological effects of kidney-protecting spirit pill.关键词
网络药理学/分子对接/保肾灵丸/糖尿病肾病Key words
network pharmacology/molecular docking/kidney-protecting spirit pill/diabetic nephropathy分类
医药卫生引用本文复制引用
刘慧娟,胡月,王安,曹富,余水红,吴其国..基于网络药理学与分子对接技术探讨保肾灵丸治疗糖尿病肾病的机制[J].沈阳医学院学报,2024,26(4):360-369,375,11.基金项目
安徽省教育厅优秀青年教师培育重点项目(No.YQYB2023120) （No.YQYB2023120）
安徽省高校自然科学研究重点项目(No.2023AH053434) （No.2023AH053434）
校级科研团队项目"中药活性物质基础及制剂研发创新团队"(No.KCTD202304) （No.KCTD202304）
