生殖医学杂志2024，Vol.33Issue(7)：860-865,6.DOI:10.3969/j.issn.1004-3845.2024.07.003
玻璃化冷冻囊胚复苏后不同体外培养时间对临床妊娠结局的影响
Effects of duration of in vitro culture on clinical pregnancy outcomes after vitrification frozen blastocyst resuscitation
摘要
Abstract
Objective:To investigate the effects of duration of in vitro culture on clinical pregnancy outcomes after thawing and resuscitation of vitrified dilated blastocyst. Methods:A retrospective analysis was conducted on 1 867 cycles of Day 5(D5)and Day 6(D6)blastocyst transfer with vitrification freezing in Reproductive Medicine Center of Northwest Women's &Children's Hospital between January 1,2021 and December 31,2022.Based on the duration of in vitro culture,women were divided into Group A(＜2 hours,n=958)and Group B(2 to 4 hours,n=909).Furthermore,based on the number of transferred blastocysts,two subgroups were formed as group A1(1 blastocyst transferred,n=843),group B1(1 blastocyst transferred,n=798)and group A2(2 blastocysts transferred,n=115),group B2(2 blastocysts transferred,n=111).Differences among the groups were compared in terms of HCG positive rate,clinical pregnancy rate,implantation rate,multiple pregnancy rate,early miscarriage rate,and final live birth rate. Results:There were no significant differences in mean age,infertile years,endometrial thickness and basic hormone levels between groups A1 and B1 as well as A2 and B2(P＞0.05).In terms of pregnancy outcomes,there were no significant differences in HCG positive rate,clinical pregnancy rate,embryo implantation rate,multiple birth rate,early miscarriage rate,ectopic pregnancy rate and live birth rate between group A1 and group B1(P＞0.05),but HCG positive rate in group A2 was significantly higher than that in group B2(91.30％vs.79.28％,P＜0.05).The clinical pregnancy rate(81.74％vs.71.17％),implantation rate(61.30％vs.54.05％)and live birth rate(67.83％vs.63.06％)in group A2 were slightly higher than those in group B2(P＞0.05).The Logistic analysis showed that＜2 hours of the duration of in vitro culture was an independent factor influencing HCG positive rate[OR=2.210,95％CI(1.028,4.752),P=0.042]in the cycles of 2 blastocysts transfer. Conclusions:Double blastocysts should be transferred within 2 hours after thawing to achieve better clinical outcomes,while there were no significant differences in clinical outcomes when single blastocyst was transferred within 4 hours after thawing.关键词
解冻囊胚移植/体外培养时间/临床结局Key words
Thawed blastocyst transfer/Duration of in vitro culture/Clinical outcome分类
医药卫生引用本文复制引用
杨喆东,李波,薛侠,李明昭,师娟子..玻璃化冷冻囊胚复苏后不同体外培养时间对临床妊娠结局的影响[J].生殖医学杂志,2024,33(7):860-865,6.基金项目
陕西省科技厅社发公关项目(2023-YBSF-034) （2023-YBSF-034）
