天津农业科学2024，Vol.30Issue(6)：7-14,8.DOI:10.3969/j.issn.1006-6500.2024.06.002
糯玉米和普通玉米籽粒蛋白差异分析
Differential Analysis of Gene Expression in Kernels of Glutinous Maize and Common Maize after Pollination
摘要
Abstract
In order to explore whether waxy properties of maize can affect the composition and content of storage protein,and to clarify the difference of gene expression between waxy maize and common maize,so as to provide more gene resources for waxy maize breed-ing.In this study,A-PAGE and transcriptome sequencing techniques were used to analyze the composition and gene expression pat-terns of seed storage proteins in common maize and waxy maize..The results showed that the grain storage protein composition of waxy maize was identical to that of common maize,and the gliadin migration rate of waxy maize and common maize was faster than wheat grain gliadin.The expression pattern of Zm00001d005793(mc1)in waxy maize was comparable to that of common maize,although its expression abundance was lower.The expression patterns of zein Zm00001D005793(mc1)in waxy maize and common maize were sim-ilar,with no significant difference discovered.The expression of zein Zm0000 1D049243(FL2)and Zm00001D048851(FL4)was consid-erably lower in waxy maize than in common maize The results indicated that the regulation pathway of waxy traits in maize may affect the expression of genes encoding grain storage proteins.In breeding,we should pay attention to the changes of waxy properties and seed storage protein,so as to enhance the diversification of breeding products and enhance market competitiveness.关键词
玉米/籽粒储藏蛋白/醇溶蛋白/加工品质Key words
maize/seed storage protein/gliadin/end-use quality分类
农业科技引用本文复制引用
陈华峰,刘颖,袁月,张晓,张家宁,刘丹..糯玉米和普通玉米籽粒蛋白差异分析[J].天津农业科学,2024,30(6):7-14,8.基金项目
天津市青年基金(小麦籽粒醇溶蛋白主效编码基因克隆及功能解析,23JCQNJC00640) （小麦籽粒醇溶蛋白主效编码基因克隆及功能解析,23JCQNJC00640）
2023年天津市农科院种业创新研究(优异鲜食玉米种质资源创制及新品种选育) （优异鲜食玉米种质资源创制及新品种选育）
河北科技师范学院科学研究基金(2024YB020) （2024YB020）
