天津农业科学2024，Vol.30Issue(6)：31-36,6.DOI:10.3969/j.issn.1006-6500.2024.06.006
草莓红中柱根腐病病原菌的鉴定和生长特性
Identification and Biological Characteristics of the Red Stele Root Rot Pathogen of Strawberry
摘要
Abstract
A variety of diseases and pests were becoming quite serious with the expanded planting area.Among of them,red stele root rot was one of the most destructive soil borne disease without efficient control strategies,which caused enormous economic losses.This disease was observed on strawberry in greenhouse in Xinkou Town,Xiqing District,Tianjin in March 2023.Large areas of strawberry became wilted and died.The xylem of the main root was found to be turned reddish brown and decayed from root cross-cutting.5 strains with consistent morphological characteristics were isolated from the root tissues of 6 strawberry diseased plants following tissue separation method.The isolated colonies were round,white,with a dark pink color on the back and a light pink color on the edge.Large conidia were sickle shaped,while small conidia were oval shaped.The pathogenicity testing based on Koch's postulates showed that the strain TN97 caused similar symptoms in the stem base and roots of strawberry plants after inoculation.The genomic DNA of the strain TN97 was extracted and PCR was carried on to amplify the rDNA-ITS and EF-1α.BLASTn comparison analysis exhibited that both ITS and EF-1α(with Genebank accession number PP257645 and PP297566)showed 100％similarity with Fusarium prolif-eratum and gathered in the same branch with F.Proliferatum based on phylogenetic analysis.The optimal carbon and nitrogen sources for TN97 growth were sucrose and potassium nitrate.The optimal growth temperature was 25℃ and the optimal growth pH of TN97 was ranged from 8 to 10.In conclusion,this is the first report of F.proliferatum causing red stele root rot on strawberry in Tianjin,which is going to shed light on disease diagnosis and effective control strategies.关键词
草莓/红中柱根腐病/层出镰孢菌/病原菌鉴定Key words
strawberry/red stele root rot/Fusarium.proliferatum/pathogens identification分类
农业科技引用本文复制引用
张蕾力,张明宇,汪宇轩,彭旭嵘,周逸文,单宏英..草莓红中柱根腐病病原菌的鉴定和生长特性[J].天津农业科学,2024,30(6):31-36,6.基金项目
国家自然科学基金青年项目(32001868) （32001868）
天津市教委科技计划项目(2021KJ105) （2021KJ105）
国家大学生创新创业培训计划(202010061009,202110061041). （202010061009,202110061041）
