天津农业科学2024，Vol.30Issue(6)：37-43,7.DOI:10.3969/j.issn.1006-6500.2024.06.007
性别与生境因素对黑线姬鼠肠道菌群多样性的影响
Effects of Sex and Habitat Factors on Intestinal Flora Diversity of Apodemus agrarius
摘要
Abstract
In order to investigate the composition and diversity of the intestinal flora of Apodemus agrarius,the effects of sex differ-ences and habitat differences on the intestinal flora of Apodemus agrarius were discussed.The 16S rRNA V3-V4 region of the intesti-nal flora of Apodemus agrarius was amplified by PCR using high-throughput sequencing technology and analyzed.The results showed that the first three dominant intestinal flora were roughly the same at phylum,order and genus level between male and female groups of Apodemus agrarius in farmland,but the proportion of each bacterial group was different.The first three dominant intestinal flora were different at phylum,order and genus level between male and female groups of Apodemus agrarius in scrub.Alpha diversity anal-ysis showed that there was no significant difference in intestinal flora between male and female of Apodemus agrarius in farmland.There was significant difference in intestinal flora diversity between male and female of Apodemus agrarius in scrub,but no significant difference in intestinal flora richness.There was no significant difference in intestinal flora of Apodemus agrarius in different habitats.ANOSIM analysis showed that there was no significant difference in intestinal flora between male and female groups of Apodemus a-grarius in farmland.The intestinal flora was significantly different between male and female of Apodemus agrarius in scrub.There was no significant difference in intestinal flora between two groups of Apodemus agrarius.In conclusion,sex and habitat have influence on the composition and diversity of intestinal flora of Apodemus agrarius.关键词
黑线姬鼠/肠道菌群/高通量测序/16S rRNAKey words
Apodemus agrarius/intestinal flora/high-throughput sequencing/16S rRNA分类
农业科技引用本文复制引用
金志民,黄笑然,李强,杨文建,贾修歧..性别与生境因素对黑线姬鼠肠道菌群多样性的影响[J].天津农业科学,2024,30(6):37-43,7.基金项目
国家重点研发计划子课题(2023YFF0724603-4) （2023YFF0724603-4）
黑龙江省2023年度省属高校基本科研业务费科研项目(1453QN029) （1453QN029）
牡丹江师范学院青年学术骨干项目(MNUQN202305) （MNUQN202305）
生物学学科卓越研究生培养模式改革创新项目(MSYZYXM2022004) （MSYZYXM2022004）
