摘要
Abstract
To optimize the extraction process of white kidney bean amylase inhibitor extract(α-AI)and explore the weight loss effect and safety of white kidney bean α-AI-L-carnitine complex,this study used the activity of white kidney bean α-AI extract as the detection indicator,and used white kidney bean powder particle size,extraction solution pH,solid-liquid ratio,extraction temperature,and extraction time as single factors.Based on these single factors,a response surface methodology was designed to optimize the ex-traction process of white kidney bean α-AI.Verify the weight loss effect and safety of the compound through weight loss function e-valuation methods and acute oral tests in rats.The results showed that the optimal extraction process for white kidney bean extract was as follows:extraction powder with a particle size of 80 mesh,extraction solution pH=6,temperature of 53 ℃,extraction time of 3.3 hours,and solid-liquid ratio of 1∶7.There was a significant difference in body weight between the control group and the model group,and no abnormalities were observed in the acute toxicity test of the compound.This study can provide new ideas and data support for the efficient extraction of white kidney bean extract and the development of weight loss formulas.关键词
白芸豆/淀粉酶抑制剂提取物/提取工艺优化/左旋肉碱/减肥功效Key words
white kidney bean extract/response surface/L-carnitine/weight loss分类
生物科学
