京尼平苷对代谢综合征大鼠的干预作用及对脂肪因子的影响OACSTPCD

Objective To explore the effect of geniposide on high fat/high fructose diet-induced metabolic syndrome(HFFD-MS)and its influence on the adipocytokines.Methods HFFD-MS models of rats were induced by a 16-week high-fat/high-fructose di-et,and were randomly divided into model group,geniposide dose groups(30,60 and 120 mg/kg,respectively),and positive control(metformin 50 mg/kg)group,while the other rats fed with ordinary diet and pure water in the same period were divided into normal group with 10 rats in each group.After 8 weeks of intragastric administration,the fat-body ratio and insulin resistance(HOMA-IR)were calculated,fasting blood glucose(FBG),total cholesterol(TC),triglyceride(TG),free fatty acid(FFA),and insulin(INS)levels in serum were detected,and the pathologic changes were observed in perirenal adipocytes.The serum levels of adipocytokine leptin(LP),visfatin(VIS),adiponectin(APN),resistin(RES),tumor necrosis factor α(TNF-α)and interleukin 6(IL-6)were detected by radioimmunoassay.Results The perirenal adipocytes in geniposide dose groups were smaller and more tightly arranged than those in the model group.Compared with the model group,geniposide administration significantly reduced the body and visceral fat weights,and fat-body ratio in HFFD-MS rats(P<0.05),significantly down-regulated FBG,HOMA-IR and serum TC,TG,FFA and INS levels(P<0.01),significantly reduced serum LP,RES,TNF-α and IL-6 levels(P<0.05 or P<0.01),and the high-dose of geniposide evidently up-regulated the expression of APN(P<0.01).Geniposide administration had no significant effect on VIS.Conclusions Geniposide can obviously improve the pathological process of HFFD-MS by regulating the abnormal secretion of adipo-kines,reducing the accumulation of visceral fat and correcting the disorder of glucose-lipid metabolism.